دورية أكاديمية
Ion Exchange and Thin Layer Chromatographic Separation and Identification of Amino Acids in a Mixture: An Experiment for General Chemistry and Biotechnology Laboratories
| العنوان: | Ion Exchange and Thin Layer Chromatographic Separation and Identification of Amino Acids in a Mixture: An Experiment for General Chemistry and Biotechnology Laboratories |
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| اللغة: | English |
| المؤلفون: | Brunauer, Linda S., Caslavka, Katelyn E., Van Groningen, Karinne |
| المصدر: | Journal of Chemical Education. Dec 2014 91(12):2216-2220. |
| الإتاحة: | Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeducTest |
| تمت مراجعته من قبل الزملاء: | Y |
| Page Count: | 5 |
| تاريخ النشر: | 2014 |
| نوع الوثيقة: | Journal Articles Reports - Descriptive |
| Education Level: | Higher Education Postsecondary Education |
| الواصفات: | Science Experiments, Laboratory Experiments, College Science, Undergraduate Students, Science Instruction, Chemistry, Biochemistry, Biotechnology, Laboratory Procedures, Color |
| DOI: | 10.1021/ed500226q |
| تدمد: | 0021-9584 |
| مستخلص: | A multiday laboratory exercise is described that is suitable for first-year undergraduate chemistry, biochemistry, or biotechnology students. Students gain experience in performing chromatographic separations of biomolecules, in both a column and thin layer chromatography (TLC) format. Students chromatographically separate amino acids (AA) in an unknown mixture using a small column of Dowex-50 resin. The AA elution profile is determined by graphical analysis of a rapid ninhydrin microplate spectrophotometric assay that converts the colorless AA into colored ninhydrin derivatives. Column fractions corresponding to elution "peaks" are further analyzed by TLC on silica gel plates alongside AA standards. Visualization of AA migration on TLC is accomplished with a ninhydrin dipping protocol. Students use their chromatography data, structural information about the resin and the various AA, and their knowledge of intermolecular attractions and acid-base chemistry to determine the identity of the AA in their unknown mixture. |
| Abstractor: | As Provided |
| Number of References: | 14 |
| Entry Date: | 2014 |
| رقم الانضمام: | EJ1048361 |
| قاعدة البيانات: | ERIC |
| تدمد: | 0021-9584 |
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| DOI: | 10.1021/ed500226q |