A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction
| العنوان: | A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction |
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| المؤلفون: | Lidén, Åsa, Karlström, Åsa, Lannergård, Jonas, Kalamajski, Sebastian, Guss, Bengt, Rubin, Kristofer, Rydén, Cecilia |
| المصدر: | Biochemical and Biophysical Research Communications - BBRC. 340(2):604-610 |
| مصطلحات موضوعية: | Adhesins, Bacterial/*metabolism/physiology, Animals, Cattle, Cell Adhesion/physiology, Cell Line, Collagen/*metabolism, Extracellular Matrix Proteins/metabolism, Gels/metabolism, Humans, Mice, Myoblasts/metabolism, Research Support, Non-U.S. Gov't, Streptococcus equi/chemistry/*metabolism, Transition Temperature, MEDICINE, MEDICIN |
| الوصف: | The N-terminal fragment (FNZN) of the fibronectin-binding protein FNZ from Streptococcus equi subspecies zooepidemicus was investigated as to effects on murine cell interactions with extracellular matrix proteins. FNZN bound to immobilized fibronectin (FN) and native, but not denatured, collagen type I. FNZN had no effect on primary adhesion of cells from the murine myoblastic C2C12 cell line to immobilized fibronectin. C2C12 cells adhered to immobilized FNZN, a process that was not inhibited by anti-human FN IgG or by an inhibitor of integrin alphaVbeta3. C2C12 cells lack collagen-binding beta1 integrins and neither adhere to native collagen nor mediate contraction of three-dimensional collagen gels. FNZN stimulated collagen gel contraction by C2C12 cells but not adhesion of C2C12 cells to collagen. Experiments with an alphaVbeta3-inhibitor suggested that FNZN promoted contraction by a process requiring alphaVbeta3. Our data suggest that FNZN by binding to cells, collagen, and FN modulate complex adhesive processes mediated by the alphaVbeta3 integrin. Since alphaVbeta3-mediated contractile events function to counteract edema formation during inflammation, it is possible that FNZN and its secreted homologue FNE modulate edema responses in infected tissues. |
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| الوصول الحر: | https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-79446Test http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=16376297&dopt=CitationTest |
| قاعدة البيانات: | SwePub |
| تدمد: | 0006291X 10902104 |
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| DOI: | 10.1016/j.bbrc.2005.12.043 |