Formulation and stabilization of an Arthrobacter strain with good storage stability and 4-chlorophenol-degradation activity for bioremediation
| العنوان: | Formulation and stabilization of an Arthrobacter strain with good storage stability and 4-chlorophenol-degradation activity for bioremediation |
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| المؤلفون: | Bjerketorp, Joakim, Röling, Wilfred F. M., Feng, Xinmei, Garcia, Armando Hernández, Heipieper, Hermann J., Håkansson, Sebastian |
| المصدر: | Applied Microbiology and Biotechnology. 102(4):2031-2040 |
| مصطلحات موضوعية: | Arthrobacter, Bioaugmentation, Bioremediation, Formulation, Microbial stabilization, Bacteria, Biodegradation, Biotechnology, Cells, Cytology, Degradation, Pollution, Strain, Bio-augmentation, Carcinogenic properties, Controlled environment, Environmental concerns, Environmental conditions, Gram-positive bacterium, Stabilization |
| الوصف: | Chlorophenols are widespread and of environmental concern due to their toxic and carcinogenic properties. Development of less costly and less technically challenging remediation methods are needed; therefore, we developed a formulation based on micronized vermiculite that, when air-dried, resulted in a granular product containing the 4-chlorophenol (4-CP)-degrading Gram-positive bacterium Arthrobacter chlorophenolicus A6. This formulation and stabilization method yielded survival rates of about 60% that remained stable in storage for at least 3 months at 4 °C. The 4-CP degradation by the formulated and desiccated A. chlorophenolicus A6 cells was compared to that of freshly grown cells in controlled-environment soil microcosms. The stabilized cells degraded 4-CP equally efficient as freshly grown cells in two different set-ups using both hygienized and non-treated soils. The desiccated microbial product was successfully employed in an outdoor pot trial showing its effectiveness under more realistic environmental conditions. No significant phytoremediation effects on 4-CP degradation were observed in the outdoor pot experiment. The 4-CP degradation kinetics from both the microcosms and the outdoor pot trial were used to generate a predictive model of 4-CP biodegradation potentially useful for larger-scale operations, enabling better bioremediation set-ups and saving of resources. This study also opens up the possibility of formulating and stabilizing also other Arthrobacter strains possessing different desirable pollutant-degrading capabilities. |
| وصف الملف: | |
| الوصول الحر: | https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-33249Test https://doi.org/10.1007/s00253-017-8706-6Test |
| قاعدة البيانات: | SwePub |
| تدمد: | 01757598 14320614 |
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| DOI: | 10.1007/s00253-017-8706-6 |