Patent
Culture chamber for biologicals
| العنوان: | Culture chamber for biologicals |
|---|---|
| Patent Number: | 7,390,653 |
| تاريخ النشر: | June 24, 2008 |
| Appl. No: | 10/725607 |
| Application Filed: | December 02, 2003 |
| مستخلص: | The culture chamber of the present invention has a fluid-filled culture compartment in which cells, tissues and other biologicals are cultured. The culture compartment is transversed by one or more molecular weight cut-off membranes attached to a membrane carrier assembly. Incoming nutrients are transported through the membrane into the culture compartment and metabolic waste products are transported away from the fluid-filled culture compartment through the membrane and out the chamber outlet. Both reusable and disposable culture chambers are described for culturing cells, cell aggregates, particles, tissues and organoids. |
| Inventors: | Akers, Roger (Houston, TX, US); Anderson, William J. (Richmond, TX, US); Dinges, Jr., Adrian F. (Houston, TX, US); Navran, Jr., Stephen S. (Houston, TX, US) |
| Assignees: | Synthecon, Inc. (Houston, TX, US) |
| Claim: | 1. A culture chamber comprising: (a) a tubular housing, wherein the housing comprises a right circular cylindrical sleeve having a first and a second end, and a first and a second end fitting including an interior projection, the interior projection having an outer diameter that sealingly fits within a bore of the sleeve, a nozzle on an exterior side of the end fitting, a counterbore in the interior projection, and a through bore passing through the end fitting, the through bore extending from the nozzle to the counterbore; (b) a growth compartment within the housing; (c) a liquid circulation system for circulating a culture medium through the growth compartment, the circulation system including a liquid inlet and a liquid outlet; and (d) a plurality of membrane carrier assembly assemblies transversing the growth compartment, wherein each membrane carrier assembly comprises a support cylinder having a first end in communication with the liquid inlet and a second end in communication with the liquid outlet, wherein the support cylinder transverses the growth compartment and wherein the support cylinder has an axial blind hole in the first and second end of the cylinder, the blind hole aligned with the through bore of the end fitting when the cylinder is positioned in the counterbore of the end fitting and wherein an interior end of the blind hole intersects a plurality of radial cross holes extending to the exterior surface of the cylinder, a molecular weight cut-off membrane secured to an exterior surface of the support cylinder, and a chamber bordered on one side by the exterior surface of the cylinder and on an opposed side by an interior surface of the membrane, the chamber containing circulating culture medium, wherein the membrane allows the diffusion of a set of biochemicals having a defined molecular weight between the growth compartment and the chamber. |
| Claim: | 2. The culture chamber of claim 1 , wherein the plurality of radial cross holes are coplanar and equispaced about the exterior surface of the cylinder. |
| Claim: | 3. The culture chamber of claim 1 , wherein each radial cross hole intersects a surface pocket on the exterior surface of the cylinder. |
| Claim: | 4. The culture chamber of claim 3 , wherein the surface pocket has an arcuate cross-section. |
| Claim: | 5. The culture chamber of claim 3 , wherein the surface pocket extends from a point of intersection with the radial cross hole to a termination point on the exterior surface of the cylinder, the termination point positioned between the point of intersection and a mid-point of a length of the cylinder. |
| Claim: | 6. A culture vessel comprising: (a) a housing having a right circular cylindrical sleeve having a first and a second end; and a first and a second end fitting including an interior projection, the interior projection having an outer diameter that sealingly fits within a bore of the sleeve to seal the first and second ends of the sleeve, a nozzle on an exterior side of the first and second end fitting, a counterbore in the interior projection, and a through bore passing through the first and second end fitting, the through bore extending from the nozzle to the counterbore; (b) a growth compartment within the bore of the sleeve; (c) a support cylinder transversing the growth compartment, the support cylinder having a first and second end, each end having a liquid channel extending from the end of the cylinder to an exterior surface of the cylinder, wherein the liquid channel includes a blind hole in the end of the cylinder that intersects a plurality of coplanar radial cross holes extending to the exterior surface of the cylinder, and a plurality of surface pockets intersected by the radial cross holes, whereby the liquid channel is in liquid communication with the through bore of the end fitting whenever the cylinder is positioned in the counterbore of the interior projection; and (d) a molecular weight cut-off membrane secured to an exterior surface of the support cylinder and overlaying the surface pockets in the exterior surface of the cylinder to provide a chamber between the exterior surface of the cylinder and an interior surface of the membrane, the chamber in liquid communication with the liquid channels of the support cylinder and the growth compartment, wherein the membrane allows the passage of a set of biochemicals having a defined molecular weight between the growth compartment and the chamber. |
| Claim: | 7. The culture chamber of claim 6 having a number of chambers transversing the growth compartment, wherein each chamber is a component of a liquid circulation system for circulating a liquid culture medium through the growth compartment. |
| Claim: | 8. A culture chamber comprising: (a) a tubular housing; (b) a growth compartment within the housing; (c) a liquid circulation system for circulating a culture medium through the growth compartment, the circulation system including a liquid inlet and a liquid outlet; (d) a plurality of membrane carrier assemblies transversing the growth compartment, each membrane carrier assembly comprising (i) a support cylinder having a first end and a second end, each end having a fluid channel comprising a blind hole in the end of the cylinder, a plurality of coplanar radial cross holes extending from the blind hole to the exterior surface of the cylinder, and a surface pocket on the exterior surface of the cylinder, wherein each surface pocket is intersected by one cross hole, and (ii) a molecular weight cut-off membrane secured to an exterior surface of the support cylinder over the surface pockets of the fluid channels, wherein the membrane defines a set of biochemicals in the circulating culture medium for which passage through the membrane is restricted, and (iii) a chamber between the exterior surface of the cylinder and an interior surface of the membrane, the chamber in fluid communication with the liquid inlet and the liquid outlet. |
| Current U.S. Class: | 4352/972 |
| Patent References Cited: | 3682318 August 1972 Rigopulos 3722694 March 1973 Agranat 4988623 January 1991 Schwartz et al. 5026650 June 1991 Schwartz et al. 5104802 April 1992 Rhodes et al. 5153131 October 1992 Wolf et al. 5153133 October 1992 Schwartz et al. 5155034 October 1992 Wolf et al. 5155035 October 1992 Schwartz et al. 5449617 September 1995 Falkenberg et al. 5576211 November 1996 Falkenberg et al. 5637477 June 1997 Spaulding et al. 5686301 November 1997 Falkenberg et al. 5998202 December 1999 Schwartz et al. 6022733 February 2000 Tam et al. 6080581 June 2000 Anderson et al. 6107055 August 2000 Bauer et al. 6228607 May 2001 Kersten et al. |
| Other References: | Rai M. et al.; Expression systems for production of heterologous proteins, Current Science, vol. 80, No. 9, May 10, 2001, pp. 1121-1128. cited by other Verma, R. et al.; Antibody engineering: Comparison of bacterial, yeast, insect and mammalian expression systems, Journal of Immunological Methods, 216, 1998, pp. 165-181. cited by other Hernaeus Instruments, Inc.; Genetic Engineering News, vol. 14, No. 12, Jun. 15, 1994. cited by other FiberCell Hollow Fiber Cell Culture Systems, Dec. 1, 2004 printout, Internet at bellcoglass.com. cited by other |
| Assistant Examiner: | Bowers, Nathan A |
| Primary Examiner: | Beisner, William H. |
| Attorney, Agent or Firm: | Hall, Elizabeth R. |
| رقم الانضمام: | edspgr.07390653 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |