التفاصيل البيبلوغرافية
| العنوان: |
NGAL FOR DIAGNOSIS OF RENAL CONDITIONS |
| Document Number: |
20120219956 |
| تاريخ النشر: |
August 30, 2012 |
| Appl. No: |
13/359772 |
| Application Filed: |
January 27, 2012 |
| مستخلص: |
Use of serum neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker, alone or in conjunction with creatinine to aid in the diagnosis of renal conditions such as acute tubular necrosis and acute renal failure, and a method and a kit for assigning a diagnosis of acute tubular necrosis or acute renal failure to a subject based on the correlation between the levels of NGAL and optionally creatinine in a sample obtained from a subject when compared to a sample obtained from a normal subject not experiencing acute tubular necrosis or acute renal failure. |
| Inventors: |
Devarajan, Prasad (Cincinnati, OH, US); BARASCH, Jonathan M. (New York, NY, US) |
| Claim: |
1. A method of assigning a diagnosis of acute tubular necrosis to a subject, comprising; a) measuring a level of NGAL in a sample obtained from said subject, b) comparing the level of NGAL in the sample from said subject with the level of NGAL in a sample from a normal subject, and; assigning a diagnosis of acute tubular necrosis when the level of NGAL in a sample obtained from said subject is greater than said normal subject. |
| Claim: |
2. The method of claim 1 wherein the level of NGAL in said subject is at least about 7 fold greater than said normal subject. |
| Claim: |
3. The method of claim 1 wherein the level of NGAL in a sample from a normal subject is about ng/mL. |
| Claim: |
4. A method of diagnosing whether a subject is experiencing acute renal failure or chronic renal failure, comprising; a) measuring the level of NGAL in a sample obtained from the subject; b) measuring the level of creatinine in a sample obtained from the subject; c) correlating the level of NGAL with the level of creatinine, and assigning a diagnosis of acute renal failure or chronic renal failure based on said correlation. |
| Claim: |
5. The method of claim 4 wherein when there is a high correlation between the measured level of NGAL and creatinine, assigning a diagnosis of acute renal failure to said subject. |
| Claim: |
6. The method of claim 4 wherein when there is no correlation between the measured level of NGAL and creatinine, assigning a diagnosis of chronic renal failure to said subject. |
| Claim: |
7. The method of claim 4 wherein the level of NGAL is measured in a sample of serum obtained from said subject. |
| Claim: |
8. The method of claim 4 wherein the level of creatinine is measured in a sample of serum obtained from said subject. |
| Claim: |
9. The method of claim 4 wherein the level of NGAL is measured in a sample of plasma obtained from said subject. |
| Claim: |
10. The method of claim 4 wherein the level of NGAL is measured in a sample of blood obtained from said subject. |
| Claim: |
11. A kit for the diagnosis of acute renal failure or chronic renal failure in a subject, comprising: a) a monoclonal antibody that recognises human NGAL; b) a tube for collecting a blood sample containing either citrate, EDTA or heparin; c) instructions for obtaining an assay result and performing a correlation of assay data to determine whether a subject has acute renal failure or chronic renal failure; wherein said instructions further indicate that a high degree of correlation between a NGAL level and creatinine level is indicative of acute renal failure; and wherein when there is no correlation between a NGAL level and creatinine level, said instructions indicate chronic renal failure is present. |
| Current U.S. Class: |
435/612 |
| Current International Class: |
01; 12 |
| رقم الانضمام: |
edspap.20120219956 |
| قاعدة البيانات: |
USPTO Patent Applications |
