التفاصيل البيبلوغرافية
| العنوان: |
ASSAY FOR THE DETECTION OF FACTORS THAT MODULATE THE EXPRESSION OF INAGP |
| Document Number: |
20090156458 |
| تاريخ النشر: |
June 18, 2009 |
| Appl. No: |
12/062740 |
| Application Filed: |
April 04, 2008 |
| مستخلص: |
A reporter construct contains mammalian INGAP 5′-regulatory region or a fragment thereof, a minimal promoter element from mammalian INGAP or a heterologous promoter, and a reporter gene. The reporter construct can be used to screen for agents which alone or in combination up-regulate or down-regulate reporter gene expression. Alternatively, the reporter construct can be used to screen for agents that bind to the hamster INGAP 5′-regulatory region or a fragment thereof. |
| Inventors: |
Taylor-Fishwick, David A. (Norfolk, VA, US); Vinik, Aaron I. (Norfolk, VA, US) |
| Assignees: |
GMP ENDOTHERAPEUTICS, INC. (Fort Lauderdale, FL, US) |
| Claim: |
1. A method for identifying agents which modulate INGAP expression, comprising: contacting a host cell comprising a reporter construct having at least one INGAP regulatory region and a region encoding a detectable product with a test agent; determining expression of the detectable product in the cell; and identifying the test agent as a modulator of INGAP expression if the test agent modulates expression of the detectable product in the cell. |
| Claim: |
2. The method of claim 1 wherein the regulatory region nucleotide sequence comprises of one or more regions chosen from nucleotides from the group consisting of SEQ ID NO: 1, 2, 23, 32, 35, 37, 28, 24, 25, 26, 27, 29, 30, 31, 33, 34, 38, and 36. |
| Claim: |
3. The method of claim 6 wherein the regulatory sequence comprises nucleotide sequences 1-3137 of SEQ ID NO: 2 |
| Claim: |
4. The method of claim 2 wherein the reporter construct, further comprises: a promoter element interposed between the regulatory region nucleotide sequence and the nucleotide sequence encoding the detectable product. |
| Claim: |
5. The method of claim 4 wherein the promoter element is selected from SEQ ID NO: 2. |
| Claim: |
6. An in vitro method for identifying agents which modulate INGAP expression, comprising: a. contacting a reporter construct having at least one INGAP regulatory region and a region encoding a detectable product with a test substance under conditions sufficient for transcription and translation of said nucleotide sequence; determining expression of the detectable protein or nucleic acid product; and identifying the test substance as a modulator of INGAP expression if the test substance modulates expression of the detectable product. |
| Claim: |
7. The method of claim 6 wherein the regulatory region nucleotide sequence comprises of one or more regions chosen from nucleotides from the group consisting of SEQ ID NO: 1, 2, 23, 32, 35, 37, 28, 24, 25, 26, 27, 29, 30, 31, 33, 34, 38, and 36. |
| Claim: |
8. The method of claim 7 wherein the regulatory sequence comprises nucleotide sequences 1-3137 of SEQ ID NO: 2 |
| Claim: |
9. The method of claim 6 wherein the reporter construct, further comprises: a promoter element interposed between the regulatory region nucleotide sequence and the nucleotide sequence encoding the detectable product. |
| Claim: |
10. The reporter construct of claim 9 wherein the promoter element is selected from SEQ ID NO: 2. |
| Claim: |
11. An in vitro method for identifying agents which modulate INGAP expression, comprising: contacting the SEQ ID NOS 1, 2, 23, 32, 35, 37, 28, 24, 25, 26, 27, 29, 30, 31, 33, 34, 38, and 36 or fragments thereof with a test agent; determining binding of the test agent to the nucleic acid; and identifying the test agent as a potential modulator of INGAP expression if the test agent binds to the nucleic acid. |
| Claim: |
12. The method of claim 11 wherein the sequence comprises nucleotide sequences 1-3137 of SEQ ID NO: 2 |
| Claim: |
13. A method for inducing INGAP expression in a mammal in need thereof, comprising administering to the mammal an effective amount of a factor that stimulates INGAP expression in the said mammal. |
| Claim: |
14. The method of claim 13 wherein the factor that stimulates INGAP expression was identified by the methods of claim 1. |
| Claim: |
15. The method of claim 14 wherein the factor that stimulates INGAP expression was identified by the methods of claim 11. |
| Claim: |
16. The method of claim 13 wherein the factor that stimulates INGAP expression is selected from hLIF or PMA |
| Current U.S. Class: |
514/2 |
| Current International Class: |
61; 12; 12 |
| رقم الانضمام: |
edspap.20090156458 |
| قاعدة البيانات: |
USPTO Patent Applications |
