دورية أكاديمية
Control of phenotypic diversification based on serial cultivations on different carbon sources leads to improved bacterial xylanase production
| العنوان: | Control of phenotypic diversification based on serial cultivations on different carbon sources leads to improved bacterial xylanase production |
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| المؤلفون: | Romain, B., Delvigne, Frank, Rémond, C., Rakotoarivonina, H. |
| المصدر: | Bioprocess and Biosystems Engineering, 45 (8), 1359 - 1370 (2022) |
| بيانات النشر: | Springer Science and Business Media Deutschland GmbH, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Lignocellulosic substrates, Phenotypic heterogeneity, Sporulation, Successive cultivations, Thermobacillus xylanilyticus, Xylanases, Bacteria, Carbon, Cytology, Enzymes, Esters, Metabolism, Straw, Substrates, Enzymatic activities, Enzyme production, Hemicellulases, Stationary phase, Successive cultivation, Thermobacillus xylanilyticu, Cells, acetylesterase, carbon, xylan 1,4 beta xylosidase, xylan endo 1,3 beta xylosidase, endo 1,4 beta xylanase, esterase, xylan, Article, carbon source, cell heterogeneity, controlled study, enzyme activity, enzyme synthesis, flow cytometry, Gram positive bacterium, nonhuman, phenotypic variation, wheat bran, bacterium, dietary fiber, metabolism, Dietary Fiber, Endo-1,4-beta Xylanases, Esterases, Xylans, Life sciences, Biotechnology, Sciences du vivant, Biotechnologie |
| الوصف: | Thermobacillus xylanilyticus is a thermophilic and hemicellulolytic bacterium of interest for the production of thermostable hemicellulases. Enzymes’ production by this bacterium is challenging, because the proliferation of a cheating subpopulation of cells during exponential growth impairs the production of xylanase after serial cultivations. Accordingly, a strategy of successive cultivations with cells transfers in stationary phase and the use of wheat bran and wheat straw as carbon sources were tested. The ratio between subpopulations and their corresponding metabolic activities were studied by flow cytometry and the resulting hemicellulases production (xylanase, acetyl esterase and β-xylosidase) followed. During serial cultivations, the results pointed out an increase of the enzymatic activities. On xylan, compared to the first cultivation, the xylanase activity increases by 7.15-fold after only four cultivations. On the other hand, the debranching activities were increased by 5.88-fold and 57.2-fold on wheat straw and by 2.77-fold and 3.34-fold on wheat bran for β-xylosidase and acetyl esterase, respectively. The different enzymatic activities then stabilized, reached a plateau and further decreased. Study of the stability and reversibility of the enzyme production revealed cell-to-cell heterogeneities in metabolic activities which could be linked to the reversibility of enzymatic activity changes. Thus, the strategy of successive transfers during the stationary phase of growth, combined with the use of complex lignocellulosic substrates as carbon sources, is an efficient strategy to optimize the hemicellulases production by T. xylanilyticus, by preventing the selection of cheaters. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501Test article peer reviewed |
| اللغة: | English |
| العلاقة: | urn:issn:1615-7591; urn:issn:1615-7605 |
| DOI: | 10.1007/s00449-022-02751-7 |
| الوصول الحر: | https://orbi.uliege.be/handle/2268/295731Test |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2Test info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsorb.295731 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1007/s00449-022-02751-7 |
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