مورد إلكتروني
Pharmacologically stimulated pupil and accommodative changes in Guinea pigs.
| العنوان: | Pharmacologically stimulated pupil and accommodative changes in Guinea pigs. |
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| المؤلفون: | Ostrin, Lisa A |
| المصدر: | Investigative ophthalmology & visual science; vol 55, iss 8, 5456-5465; 0146-0404 |
| بيانات النشر: | eScholarship, University of California 2014-08-01 |
| تفاصيل مُضافة: | Ostrin, Lisa A Garcia, Mariana B Choh, Vivian Wildsoet, Christine F |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | PurposeThe guinea pig is being used increasingly as a model of human myopia. As accommodation may influence the effects of manipulations used in experimental myopia models, understanding the accommodative ability of guinea pigs is important. Here, nonselective muscarinic agonists were used as pharmacological tools to study guinea pig accommodation.MethodsMeasurements were made on 15 pigmented guinea pigs. For in vivo testing, animals were anesthetized and, following baseline measurements, 2% pilocarpine was applied topically. Measurements included A-scan ultrasonography, optical coherence tomography (OCT) imaging, corneal topography, and refraction. In vitro lens scanning experiments were performed using anterior segment preparations, with measurements before and during exposure to carbachol. Anterior segment structures were examined histologically and immunohistochemistry was done to characterize the muscarinic receptor subtypes present.ResultsIn vivo, pilocarpine induced a myopic shift in refractive error coupled to a small, but consistent decrease in anterior chamber depth (ACD), a smaller and more variable increase in lens thickness, and a decrease in pupil size. Lens thickness increases were short-lived (10 minutes), while ACD and pupil size decreased over 20 minutes. Corneal curvature was not significantly affected. Carbachol tested on anterior segment preparations in vitro was without effect on lens back vertex distance, but did stimulate pupil constriction. Immunohistochemistry indicated the presence of muscarinic receptor subtypes 1 to 5 in the iris and ciliary body.ConclusionsThe observed pilocarpine-induced changes in ACD, lens thickness, and refraction are consistent with active accommodation in the guinea pig, through cholinergic muscarinic stimulation. |
| مصطلحات الفهرس: | Anterior Chamber, Lens, Crystalline, Pupil, Animals, Guinea Pigs, Disease Models, Animal, Pilocarpine, Muscarinic Agonists, Tomography, Optical Coherence, Corneal Topography, Refraction, Ocular, Accommodation, accommodation, anterior segment, guinea pig, muscarinic agonists, pupil size, Eye Disease and Disorders of Vision, Biological Sciences, Medical and Health Sciences, Ophthalmology & Optometry, article |
| URL: | |
| الإتاحة: | Open access content. Open access content public |
| ملاحظة: | application/pdf Investigative ophthalmology & visual science vol 55, iss 8, 5456-5465 0146-0404 |
| أرقام أخرى: | CDLER oai:escholarship.org:ark:/13030/qt026107tg qt026107tg https://escholarship.org/uc/item/026107tgTest https://escholarship.orgTest/ 1377971187 |
| المصدر المساهم: | UC MASS DIGITIZATION From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1377971187 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |