مورد إلكتروني
Glyoxalase I activity affects Arabidopsis sensitivity to ammonium nutrition
| العنوان: | Glyoxalase I activity affects Arabidopsis sensitivity to ammonium nutrition |
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| بيانات النشر: | Umeå universitet, Institutionen för fysiologisk botanik Umeå universitet, Umeå Plant Science Centre (UPSC) Department of Plant Bioenergetics, Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw, Poland Department of Plant Bioenergetics, Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw, Poland Department of Plant Bioenergetics, Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw, Poland Department of Plant Bioenergetics, Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw, Poland 2022 |
| تفاصيل مُضافة: | Borysiuk, Klaudia Ostaszewska-Bugajska, Monika Kryzheuskaya, Katsiaryna Gardeström, Per Szal, Bożena |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Key message: Elevated methylglyoxal levels contribute to ammonium-induced growth disorders in Arabidopsis thaliana. Methylglyoxal detoxification pathway limitation, mainly the glyoxalase I activity, leads to enhanced sensitivity of plants to ammonium nutrition. Abstract: Ammonium applied to plants as the exclusive source of nitrogen often triggers multiple phenotypic effects, with severe growth inhibition being the most prominent symptom. Glycolytic flux increase, leading to overproduction of its toxic by-product methylglyoxal (MG), is one of the major metabolic consequences of long-term ammonium nutrition. This study aimed to evaluate the influence of MG metabolism on ammonium-dependent growth restriction in Arabidopsis thaliana plants. As the level of MG in plant cells is maintained by the glyoxalase (GLX) system, we analyzed MG-related metabolism in plants with a dysfunctional glyoxalase pathway. We report that MG detoxification, based on glutathione-dependent glyoxalases, is crucial for plants exposed to ammonium nutrition, and its essential role in ammonium sensitivity relays on glyoxalase I (GLXI) activity. Our results indicated that the accumulation of MG-derived advanced glycation end products significantly contributes to the incidence of ammonium toxicity symptoms. Using A. thaliana frostbite1 as a model plant that overcomes growth repression on ammonium, we have shown that its resistance to enhanced MG levels is based on increased GLXI activity and tolerance to elevated MG-derived advanced glycation end-product (MAGE) levels. Furthermore, our results show that glyoxalase pathway activity strongly affects cellular antioxidative systems. Under stress conditions, the disruption of the MG detoxification pathway limits the functioning of antioxidant defense. However, under optimal growth conditions, a defect in the MG detoxification route results in the activation of antioxidative systems. |
| مصطلحات الفهرس: | Ammonium nutrition, d-Lactate dehydrogenase, Dicarbonyl stress, Glyoxalase, Methylglyoxal, Mitochondrial Complex I mutant, Botany, Botanik, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1007.s00299-022-02931-5 |
| URL: | Plant Cell Reports, 0721-7714, 2022, 41, s. 2393-2413 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | UPE oai:DiVA.org:umu-200516 0000-0001-5900-7395 doi:10.1007/s00299-022-02931-5 PMID 36242617 ISI:000869198900001 Scopus 2-s2.0-85139934614 1370103714 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1370103714 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1007.s00299-022-02931-5 |
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