مورد إلكتروني
Primate-specific RFPL1 gene controls cell-cycle progression through cyclin B1/Cdc2 degradation.
| العنوان: | Primate-specific RFPL1 gene controls cell-cycle progression through cyclin B1/Cdc2 degradation. |
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| المصدر: | Cell death and differentiation, 18 (2 |
| بيانات النشر: | 2011-02 |
| تفاصيل مُضافة: | Bonnefont, Jérôme Laforge, T Plastre, O Beck, Benjamin Sorce, S Dehay, Colette Krause, K-H |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Ret finger protein-like 1 (RFPL1) is a primate-specific target gene of Pax6, a key transcription factor for pancreas, eye and neocortex development. However, its cellular activity remains elusive. In this article, we report that Pax6-elicited expression of the human (h)RFPL1 gene in HeLa cells can be enhanced by in vivo p53 binding to its promoter and therefore investigated the hypothesis that hRFPL1 regulates cell-cycle progression. Upon expression in these cells, hRFPL1 decreased cell number through a kinase-dependent mechanism as PKC activates and Cdc2 inhibits hRFPL1 activity. hRFPL1 antiproliferative activity led to an increased cell population in G(2)/M phase and specific cyclin B1 and Cdc2 downregulations, which were precluded by a proteasome inhibitor. Specifically, cytoplasm-localized hRFPL1 prevented cyclin B1 and Cdc2 accumulation during interphase. Consequently, cells showed a delayed entry into mitosis and cell-cycle lengthening resulting from a threefold increase in G(2) phase duration. Given previous reports that RFPL1 is expressed during cell differentiation, its impact on cell-cycle lengthening therefore provides novel insights into primate-specific development. Journal Article Research Support, Non-U.S. Gov't SCOPUS: ar.j info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences exactes et naturelles, Sciences bio-médicales et agricoles, Animals, Carrier Proteins -- genetics -- metabolism -- physiology, Cell Division, Cyclin B -- metabolism, Cyclin B1 -- metabolism, Eye Proteins -- metabolism, G2 Phase, HeLa Cells, Homeodomain Proteins -- metabolism, Humans, Interphase, Mitosis, Paired Box Transcription Factors -- metabolism, Primates -- metabolism, Promoter Regions, Genetic, Protein Binding, Protein Kinase C -- metabolism, Repressor Proteins -- metabolism, Tumor Suppressor Protein p53 -- metabolism, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 1 full-text file(s): application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/137557 uri/info:doi/10.1038/cdd.2010.102 uri/info:pii/cdd2010102 uri/info:pmid/20725088 uri/info:scp/78651339945 uri/info:pmcid/PMC3131898 1363728195 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1363728195 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |