مورد إلكتروني
A Miniaturized Imaging Window to Quantify Intravital Tissue Regeneration within a 3D Microscaffold in Longitudinal Studies
| العنوان: | A Miniaturized Imaging Window to Quantify Intravital Tissue Regeneration within a 3D Microscaffold in Longitudinal Studies |
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| بيانات النشر: | Wiley-VCH Verlag GmbH & Co. KGaA country:DE 2022 |
| تفاصيل مُضافة: | Conci, C Jacchetti, E Sironi, L Gentili, L Cerullo, G Osellame, R Chirico, G Raimondi, M Conci C. Jacchetti E. Sironi L. Gentili L. Cerullo G. Osellame R. Chirico G. Raimondi M. T. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The biocompatibility assessment of biomaterials or the dynamic response of implanted constructs entails inflammatory events primary reflected in cell behavior at the microcirculatory system. Current protocols are based on histopathology which are over 40 years old and require the sacrifice of a huge number of laboratory animal with an unsustainable ethical burden of animal research. Intravital microscopy techniques are actually used to study implantation outcomes in real time. However, no device providing a specific tracking geometry to reposition the field of view of the microscope, for repeated analyses, exists yet. The synthetic photoresist SZ2080 is characterized here, allowing the development and in vivo validation of a miniaturized imaging window, the Microatlas, that, fabricated via two-photon polymerization, is implanted in living chicken embryos and imaged by fluorescence microscopy 3 and 4 days after the implant. The characterization of their elastomechanical and fluorescence properties highlights planar raster spacing as the most important parameter in tuning the mechanical and spectroscopic features of the structures. The quantification of cell infiltration inside the Microatlas demonstrates its potential as novel scaffold for tissue regeneration and as beacon for 3D repositioning of the microscope field of view and correction of optical aberrations. |
| مصطلحات الفهرس: | 3D-microstructured scaffold, confocal microscopy, elastomechanic, ex ovo implant, in vivo implant, intravital imaging window, two-photon imaging, two-photon polymerization, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/wos/WOS:000757692400001 volume:10 issue:7 journal:ADVANCED OPTICAL MATERIALS |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | English |
| أرقام أخرى: | ITBAO oai:boa.unimib.it:10281/359864 10.1002/adom.202101103 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85124709556 1308944086 |
| المصدر المساهم: | BICOCCA OPEN ARCH From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1308944086 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |