مورد إلكتروني
Podocalyxin is a key negative regulator of human endometrial epithelial receptivity for embryo implantation.
| العنوان: | Podocalyxin is a key negative regulator of human endometrial epithelial receptivity for embryo implantation. |
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| بيانات النشر: | NLM (Medline) United Kingdom 2021-04-15 |
| تفاصيل مُضافة: | Vollenhoven B. Lim R. Rombauts L.J. Nie G. Paule S.G. Heng S. Samarajeewa N. Li Y. Mansilla M. Webb A.I. Nebl T. Young S.L. Lessey B.A. Hull M.L. Scelwyn M. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | STUDY QUESTION: How is endometrial epithelial receptivity, particularly adhesiveness, regulated at the luminal epithelial surface for embryo implantation in the human? SUMMARY ANSWER: Podocalyxin (PCX), a transmembrane protein, was identified as a key negative regulator of endometrial epithelial receptivity; specific downregulation of PCX in the luminal epithelium in the mid-secretory phase, likely mediated by progesterone, may act as a critical step in converting endometrial surface from a non-receptive to an implantation-permitting state. WHAT IS KNOWN ALREADY: The human endometrium must undergo major molecular and cellular changes to transform from a non-receptive to a receptive state to accommodate embryo implantation. However, the fundamental mechanisms governing receptivity, particularly at the luminal surface where the embryo first interacts with, are not well understood. A widely held view is that upregulation of adhesion-promoting molecules is important, but the details are not well characterized. STUDY DESIGN, SIZE, DURATION: This study first aimed to identify novel adhesion-related membrane proteins with potential roles in receptivity in primary human endometrial epithelial cells (HEECs). Further experiments were then conducted to determine candidates' in vivo expression pattern in the human endometrium across the menstrual cycle, regulation by progesterone using cell culture, and functional importance in receptivity using in vitro human embryo attachment and invasion models. PARTICIPANTS/MATERIALS, SETTING, METHODS: Primary HEECs (n=9) were isolated from the proliferative phase endometrial tissue, combined into three pools, subjected to plasma membrane protein enrichment by ultracentrifugation followed by proteomics analysis, which led to the discovery of PCX as a novel candidate of interest. Immunohistochemical analysis determined the in vivo expression pattern and cellular localization of PCX in the human endometrium across the menstrual cycle (n=23). |
| مصطلحات الفهرس: | nidation, polymerase chain reaction, protein expression, protein function, proteomics, rank sum test, statistical significance, trophoblast, tumor spheroid, ultracentrifugation, upregulation, Western blotting, biological marker, endogenous compound, estradiol, hormone, membrane protein, podocalyxin, progesterone, in vivo study, nonhuman, analysis of variance, animal cell, animal experiment, animal model, animal tissue, article, Australia, cell membrane, cell viability, cellular distribution, conflict of interest, controlled study, down regulation, embryo, endothelium cell, female, fertility preservation, funding, gene overexpression, human, human embryo, immunohistochemistry, medical director, menstrual cycle, monolayer culture, Article |
| URL: | Click here for full text options LibKey Link |
| الإتاحة: | Open access content. Open access content This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine |
| أرقام أخرى: | AUSHL oai:repository.monashhealth.org:1/26560 Human reproduction (Oxford, England). 36 (5) (pp 1353-1366), 2021. Date of Publication: 20 Apr 2021. 1460-2350 (electronic) https://repository.monashhealth.org/monashhealthjspui/handle/1/26560Test Monash Health 33822049 [http://www.ncbi.nlm.nih.gov/pubmed/?term=33822049Test] 634754139 (Paule, Heng, Samarajeewa, Li, Mansilla, Nie) Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, Australia (Heng, Samarajeewa, Li, Mansilla, Nie) Implantation and Pregnancy Research Laboratory, School of Health and Biomedical Sciences, RMIT University, VIC, Australia (Webb, Nebl) Advance Technology and Biology Division, The Walter and Eliza Hall Institute, Parkville, VIC, Australia (Young) Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, United States (Lessey) Department of Obstetrics and Gynecology, Greenville Health System, SC, Greenville, United States (Hull) Robinson Research Institute, University of Adelaide, SA, Adelaide, Australia (Scelwyn) VICAustralia (Lim) The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC, Australia (Lim, Vollenhoven, Rombauts) Department of Obstetrics and Gynaecology, Monash University, Clayton, VIC, Australia (Vollenhoven, Rombauts) Womens and Newborn Programme, Monash Health, Clayton, VIC, Australia (Paule, Heng, Samarajeewa, Li, Mansilla, Nie) Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, Australia (Heng, Samarajeewa, Li, Mansilla, Nie) Implantation and Pregnancy Research Laboratory, School of Health and Biomedical Sciences, RMIT University, VIC, Australia (Webb, Nebl) Advance Technology and Biology Division, The Walter and Eliza Hall Institute, Parkville, VIC, Australia (Young) Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, United States (Lessey) Department of Obstetrics and Gynecology, Greenville Health System, SC, Greenville, United States (Hull) Robinson Research Institute, University of Adelaide, SA, Adelaide, Australia (Scelwyn) VICAustralia (Lim) The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC, Australia (Lim, Vollenhoven, Rombauts) Department of Obstetrics and Gynaecology, Monash University, Clayton, VIC, Australia (Vollenhoven, Rombauts) Womens and Newborn Programme, Monash Health, Clayton, VIC, Australia 1305127428 |
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| رقم الانضمام: | edsoai.on1305127428 |
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