مورد إلكتروني
Slipdisc : A versatile sample preparation platform for point of care diagnostics
| العنوان: | Slipdisc : A versatile sample preparation platform for point of care diagnostics |
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| بيانات النشر: | KTH, Proteomik och nanobioteknologi KTH, Science for Life Laboratory, SciLifeLab KTH, Fiberteknologi KTH, Wallenberg Wood Science Center KTH, Fiber- och polymerteknologi Royal Society of Chemistry 2017 |
| تفاصيل مُضافة: | Banerjee, Indradumna Salih, Tagrid Ramachandraiah, Harisha Erlandsson, Johan Pettersson, Torbjörn Araújo, A. C. Karlsson, M. Russom, Aman |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | We report a microfluidic sample preparation platform called "Slipdisc" based on slipchip technology. Slipdisc is a rotational slipchip that uses a unique hand-wound clockwork mechanism for precise movement of specially fabricated polycarbonate discs. In operation, the microchannels and microchambers carved on the closely aligned microfluidic discs convert from continuous filled paths to defined compartments using the slip movement. The clockwork mechanism introduced here is characterised by a food dye experiment and a conventional HRP TMB reaction before measuring lactate dehydrogenase (LDH) enzyme levels, which is a crucial biomarker for neonatal diagnostics. The colorimetry based detection of LDH was performed with an unmodified camera and an image analysis procedure based on normalising images and observing changes in red channel intensity. The analysis showed a close to unity coefficient of determination (R2 = 0.96) in detecting the LDH concentration when compared with a standard Chemical Analyser, demonstrating the excellent performance of the slipdisc platform with colorimetric detection. The versatile point of care sample preparation platform should ideally be suited for a multitude of applications at resource-limited settings. QC 20171201 |
| مصطلحات الفهرس: | Chemical analysis, Chemical detection, Clocks, Colorimetric analysis, Colorimetry, Coefficient of determination, Colorimetric detection, Lactate dehydrogenase, Micro-chambers, Point of care, Point of care diagnostic, Red channels, Sample preparation, Microfluidics, Analytical Chemistry, Analytisk kemi, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1039.c7ra05209j |
| URL: | RSC Advances, 2017, 7:56, s. 35048-35054 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:kth-219077 0000-0001-5199-0663 0000-0003-1874-2187 0000-0002-5444-7276 0000-0002-0242-358X doi:10.1039/c7ra05209j ISI:000405811400015 Scopus 2-s2.0-85025080747 1234964401 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1234964401 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1039.c7ra05209j |
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