مورد إلكتروني

Razgradnja celuloze i hemiceluloze pomoću bakterija iz buraga

التفاصيل البيبلوغرافية
العنوان: Razgradnja celuloze i hemiceluloze pomoću bakterija iz buraga
عناروين إضافية: Potential of Selected Rumen Bacteria for Cellulose and Hemicellulose Degradation
المصدر: Food Technology and Biotechnology; ISSN 1330-9862 (Print); ISSN 1334-2606 (Online); Volume 52; Issue 2
بيانات النشر: Faculty of Food Technology and Biotechnology, University of Zagreb 2014
تفاصيل مُضافة: Maša Zorec
Maša Vodovnik
Romana Marinšek-Logar
نوع الوثيقة: Electronic Resource
مستخلص: U probavnom se sustavu biljojeda nalaze vrlo učinkoviti mikroorganizmi što razgrađuju celulolozu i hemicelulozu iz sažvakanog biljnog materijala, te ih opskrbljuju hranjivim tvarima. Osim protozoa i gljivica, razgradnji otporne (hemi)celulozne biomase u buragu znatno pridonose i bakterije. U fokusu je ovog preglednog rada opis enzimskog sustava triju predstavnika bakterija iz buraga što proizvode celulazu, i to: Ruminococcus flavefaciens, Prevotella bryantii i Pseudobutyrivibrio xylanivorans. R. flavefaciens je poznat po proizvodnji jedne od najkompleksnijih enzimskih struktura, pa bi se mogao upotrijebiti za dizajn enzimskog kompleksa za razgradnju stanične stijenke biljaka. S druge strane, bakterije Prevotella bryantii i Pseudobutyrivibrio xylanivorans proizvode jednostavne, slobodne i vrlo aktivne ksilanaze. Bakterija P. xylanivorans ima i probiotička svojstva, pa se može upotrijebiti za proizvodnju bioplina i kao dodatak krmivu. Ispitivanje je genoma i proteoma bakterija što razgrađuju celulozu i hemicelulozu usmjereno na identifikaciju novih enzima, koji se nakon kloniranja i ekspresije u odgovarajućim domaćinima mogu upotrijebiti za izradu vrlo aktivnih rekombinantnih hidrolitičkih mikroorganizama, te primijeniti u različitim biotehnološkim procesima.
Herbivorous animals harbour potent cellulolytic and hemicellulolytic microorganisms that supply the host with nutrients acquired from degradation of ingested plant material. In addition to protozoa and fungi, rumen bacteria contribute a considerable part in the breakdown of recalcitrant (hemi)cellulosic biomass. The present review is focused on the enzymatic systems of three representative fibrolytic rumen bacteria, namely Ruminococcus flavefaciens, Prevotella bryantii and Pseudobutyrivibrio xylanivorans. R. flavefaciens is known for one of the most elaborated cellulosome architectures and might represent a promising candidate for the construction of designer cellulosomes. On the other hand, Prevotella bryantii and Pseudobutyrivibrio xylanivorans produce multiple free, but highly efficient xylanases. In addition, P. xylanivorans was also shown to have some probiotic traits, which makes it a promising candidate not only for biogas production, but also as an animal feed supplement. Genomic and proteomic analyses of cellulolytic and hemicellulolytic bacterial species aim to identify novel enzymes, which can then be cloned and expressed in adequate hosts to construct highly active recombinant hydrolytic microorganisms applicable for different biotechnological tasks.
مصطلحات الفهرس: Ruminococcus flavefaciens; Prevotella bryantii; Pseudobutyrivibrio xylanivorans; multienzimski kompleksi; ksilanaza; probiotici, Ruminococcus flavefaciens; Prevotella bryantii; Pseudobutyrivibrio xylanivorans; cellulosome; xylanase; probiotics, text, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion
URL: https://hrcak.srce.hr/122346Test
https://hrcak.srce.hr/file/180846Test
https://hrcak.srce.hr/file/180845Test
الإتاحة: Open access content. Open access content
info:eu-repo/semantics/openAccess
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ملاحظة: application/pdf
English
Croatian
أرقام أخرى: HRCAK oai:hrcak.srce.hr:122346
985289919
المصدر المساهم: HRCAK PORTAL ZNANSTVENIH CASOPISA REPUB
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رقم الانضمام: edsoai.ocn985289919
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