مورد إلكتروني
Assessment of the relative contribution of cellular components to the acetowhitening effect in cell cultures and suspensions using elastic light-scattering spectroscopy
| العنوان: | Assessment of the relative contribution of cellular components to the acetowhitening effect in cell cultures and suspensions using elastic light-scattering spectroscopy |
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| بيانات النشر: | 2007 |
| تفاصيل مُضافة: | Wu, Tao T. Qu, Jianan Y. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | We aim to investigate the mechanism of acetowhitening upon which the colposcopic diagnosis of cervical cancer is based. The changes in light scattering induced by acetic acid in intact cervical cancer cells and cellular components were studied using elastic light-scattering spectroscopy. After adding acetic acid to intact cancer cell culture samples (cell suspensions and attached monolayer cell cultures), a slight decrease in small-angle forward scattering was observed, while the large-angle scattering increased by a factor of 5-9, indicating that acetowhitening signals are mainly contributed from small-sized intracellular scattering structures. The cellular components of different sizes and masses were isolated to investigate their individual contribution to the changes of light scattering induced by acetic acid. The study provided the evidence that the cellular components of diameter smaller than 0.2 mu m in the cytoplasm are the major contributors to the acetowhitening effect in whole cells, while the light scattering from the mitochondria are not sensitive to the acetic acid. (c) 2007 Optical Society of America. |
| مصطلحات الفهرس: | Acetic Acid: diagnostic use, Acetic Acid: pharmacology, Carcinoma, Squamous Cell: pathology, Carcinoma, Squamous Cell: physiopathology, Cell Line, Tumor, Computer Simulation, Humans, Models, Biological, Spectrum Analysis: methods, Subcellular Fractions: drug effects, Subcellular Fractions: pathology, Article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | English |
| أرقام أخرى: | HNK oai:repository.ust.hk:1783.1-29463 Applied optics, v. 46, (21), 2007, JUL 20, p. 4834-4842 0003-6935 https://doi.org/10.1364/AO.46.004834Test 898136315 |
| المصدر المساهم: | HONG KONG UNIV OF SCI & TECH, THE From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.ocn898136315 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |