مورد إلكتروني
Knockdown of ki-67 by dicer-substrate small interfering RNA sensitizes bladder cancer cells to curcumin-induced tumor inhibition.
| العنوان: | Knockdown of ki-67 by dicer-substrate small interfering RNA sensitizes bladder cancer cells to curcumin-induced tumor inhibition. |
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| المصدر: | Department of Pathology, Anatomy, and Cell Biology Faculty Papers |
| بيانات النشر: | Jefferson Digital Commons 2012-01-01T08:00:00Z |
| تفاصيل مُضافة: | Pichu, Sivakamasundari Krishnamoorthy, Swapna Shishkov, Andrei Zhang, Bi McCue, Peter Ponnappa, Biddanda C |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Transitional cell carcinoma (TCC) of the urinary bladder is the most common cancer of the urinary tract. Most of the TCC cases are of the superficial type and are treated with transurethral resection (TUR). However, the recurrence rate is high and the current treatments have the drawback of inducing strong systemic toxicity or cause painful cystitis. Therefore, it would be of therapeutic value to develop novel concepts and identify novel drugs for the treatment of bladder cancer. Ki-67 is a large nucleolar phosphoprotein whose expression is tightly linked to cell proliferation, and curcumin, a phytochemical derived from the rhizome Curcuma longa, has been shown to possess powerful anticancer properties. In this study, we evaluated the combined efficacy of curcumin and a siRNA against Ki-67 mRNA (Ki-67-7) in rat (AY-27) and human (T-24) bladder cancer cells. The anticancer effects were assessed by the determination of cell viability, apoptosis and cell cycle analysis. Ki-67-7 (10 nM) and curcumin (10 µM), when treated independently, were moderately effective. However, in their combined presence, proliferation of bladder cancer cells was profoundly (>85%) inhibited; the rate of apoptosis in the combined presence of curcumin and Ki-67-7 (36%) was greater than that due to Ki-67-7 (14%) or curcumin (13%) alone. A similar synergy between curcumin and Ki-67-7 in inducing cell cycle arrest was also observed. Western blot analysis suggested that pretreatment with Ki-67-7 sensitized bladder cancer cells to curcumin-mediated apoptosis and cell cycle arrest by p53- and p21-independent mechanisms. These data suggest that a combination of anti-Ki-67 siRNA and curcumin could be a viable treatment against the proliferation of bladder cancer cells. |
| مصطلحات الفهرس: | animal cell; antineoplastic activity; antiproliferative activity; apoptosis; article; bladder cancer; cancer cell; cancer cell culture; cancer inhibition; cell cycle; cell cycle arrest; cell proliferation; cell viability; concentration response; controlled study; drug efficacy; drug potentiation; drug sensitization; gene expression; genetic transfection; human; human cell; Ki 67 gene; nonhuman; protein expression; rat; real time polymerase chain reaction; Western blotting; curcumin; dicer substrate small interfering RNA; Ki 67 antigen; messenger RNA; small interfering RNA; unclassified drug, Medical Anatomy, Medical Cell Biology, Medical Pathology, article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | application/pdf |
| أرقام أخرى: | TVJ oai:jdc.jefferson.edu:pacbfp-1101 857634831 |
| المصدر المساهم: | THOMAS JEFFERSON UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.ocn857634831 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |