مورد إلكتروني
Secretory characteristics and viability of human term placental tissue after overnight cold preservation.
| العنوان: | Secretory characteristics and viability of human term placental tissue after overnight cold preservation. |
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| المصدر: | Human reproduction, 15 (4 |
| بيانات النشر: | 2000-04 |
| تفاصيل مُضافة: | Cirelli, N Lebrun, Philippe Gueuning, C Moens, A Delogne-Desnoeck, J Dictus-Vermeulen, C Vanbellinghen, Anne-Marie Meuris, Sylvain |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Collection of human term placentae for research purposes is generally limited during working hours. Preserving placental tissue overnight might help to postpone experiments and, by extent, to increase material availability. In this study, fragments from normal placentae were incubated at 37 degrees C either immediately after delivery or after preservation at 4 degrees C in a HEPES-buffered solution or in a Roswell Park Memorial Institute (RPMI) 1640 culture medium. Protein, human chorionic gonadotrophin (HCG), human placental lactogen (HPL) and lactate dehydrogenase (LDH) contents within preserved explants were similar to those within freshly delivered ones. In contrast, HCG and HPL amounts released during incubation of preserved tissue were lower than with freshly delivered tissue. Differences were significant only during the first 3 h of incubation. Hormone releases were similarly Ca(2+)-stimulated, and Co(2+)- and low temperature-inhibited in preserved and freshly delivered tissues. After preservation, LDH leakage was also reduced. Furthermore, before and after 37 degrees C incubation during 6 h, preserved tissue was morphologically indistinguishable from freshly delivered tissue and showed neither higher incidence of DNA fragmentation, nor elevated caspase-3 activity, both of which are markers of apoptosis. This study validates an original, useful and rapid method to preserve placental tissue. Consequently, this preservation model may facilitate the study of physiological processes regulating placental hormone secretion in normal and pathological conditions. Journal Article Research Support, Non-U.S. Gov't SCOPUS: ar.j info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Apoptosis, Buffers, Calcium -- pharmacology, Caspase 3, Caspases -- analysis, Chorionic Gonadotropin -- analysis, Chorionic Gonadotropin -- secretion, Cobalt -- pharmacology, Cold Temperature, Culture Media, DNA Fragmentation, Female, Humans, In Situ Nick-End Labeling, L-Lactate Dehydrogenase -- analysis, L-Lactate Dehydrogenase -- secretion, Placenta -- drug effects, Placenta -- physiology, Placenta -- secretion, Placental Lactogen -- analysis, Placental Lactogen -- secretion, Pregnancy, Proteins -- analysis, Time Factors, Tissue Preservation, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/61999 uri/info:pmid/10739815 uri/info:scp/0034108708 764603367 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.ocn764603367 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |