رسالة جامعية
Proteomic Analysis of Human Hepatoma G2 Cells Following Treatment with Heterocyclic Amines Trp-P-1
العنوان: | Proteomic Analysis of Human Hepatoma G2 Cells Following Treatment with Heterocyclic Amines Trp-P-1 |
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العنوان البديل: | 以蛋白質體學探討雜環胺Trp-P-1處理人類肝癌細胞株HepG2 |
المؤلفون: | Da-Wei Huang, 黃大維 |
مرشدي الرسالة: | 張鴻民 |
سنة النشر: | 2005 |
المجموعة: | National Digital Library of Theses and Dissertations in Taiwan |
الوصف: | 93 Heterocyclic amines have been proved to be highly mutagenic and carcinogenic due to the activation of cytochrome P-450 in vivo. Among them, Trp-P-1 is one of the potent toxic heterocyclic amines. In the present study, proteomics on the protein expression of HepG2 cells following treatment with Trp-P-1 were conducted. In vitro, the growth inhibition of HepG2 cells increased in a time-dependent manner after treatment with Trp-P-1 at a level higher than 2 µg/mL. More than 90 % cells were inhibited when treated with 3, 4 or 5 µg/mL Trp-P-1 for 3 days. In proteomic analysis, 6, 9, and 11 protein spots were observed to be increased in quantity by more than 50 % if HepG2 cells were treated with 2 µg/mL Trp-P-1 for 1, 2, and 3 days, respectively. However, protein expression decreased in quantity by more than 50 % were determined to be 3 and 4 protein spots treated with the same amount of Trp-P-1 for 2 and 3 days, respectively. These protein spots were in gel digested by trypsin and analyzed by matrix assisted laser desorption ionization – time of flight ( MALDI-TOF ) mass spectrometry and 4, 10, and 13 proteins were successfully identified in day-1, -2, and -3 sample, respectively. Internet database ( Swiss-Prot ) search indicated that the changes in cell physiology induced by Trp-P-1 were mainly due to the influence of protein functions on energy metabolism cycle and protein structure maintain. |
Original Identifier: | 093NTU05252015 |
نوع الوثيقة: | 學位論文 ; thesis |
وصف الملف: | 101 |
الإتاحة: | http://ndltd.ncl.edu.tw/handle/22871860377729290768Test |
رقم الانضمام: | edsndl.TW.093NTU05252015 |
قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
الوصف غير متاح. |