رسالة جامعية
Molecular cloning and functional analysis of MADS box genes in monoecious plant Zucchini squash
| العنوان: | Molecular cloning and functional analysis of MADS box genes in monoecious plant Zucchini squash |
|---|---|
| العنوان البديل: | 雌雄同株異花植物矮南瓜(Zucchunusquash)中MADSBox基因之分子選殖與功能分析 |
| المؤلفون: | Yan-Ling Shi, 施燕玲 |
| مرشدي الرسالة: | Chang-Hsien Yang, 楊長賢 |
| سنة النشر: | 2001 |
| المجموعة: | National Digital Library of Theses and Dissertations in Taiwan |
| الوصف: | 89 The formation of unisexual flowers in Zucchini squash, a monoecious plant, results from the suppression of either male or female organs during flower development. Since MADS box genes play the central role for flower development, it is intriguing to investigate of the relationship between MADS box genes and the sex-determined mechanism. For this, two cDNAs showing high homology to floral organ identity MADS box genes were isolated and characterized from Z. squash. ZuAG (Zucchini AG) encodes a 230-amino acid protein that showed 92% identity and 96% similarity to CUM1, an AGAMOUS (AG) homologue in cucumber (Cucumis sativus). ZuAGL2 (Zucchini AGL2) encodes a 246-amino acid protein that showed 90% identity and 91% similarity to CAGL2, an AGL2 homologue in cucumber. Result of Southern blot analysis indicated that both ZuAG and ZuAGL2 DNA sequences are present in Z. squash genome in a single copy manner. ZuAG and ZuAGL2 mRNA were detected in floral buds of different stages but absent in vegetative leaf, suggesting that the expression of these two genes is floral specific. The expression of ZuAG was restricted in the stamen of a male flower and the carpel of a female flower; whereas ZuAGL2 was expressed in all four floral whorls. These data indicate that ZuAG and ZuAGL2 may act independently from the formation of unisexual flowers in Z. squash. Ectopic expression of ZuAG in transgenic Arabidopsis plants showed novel phenotypes with early flowering and curly leaves. In flowers, homeotic conversion of sepals to carpelloid and petal to stamen-like structures were observed in 35S::ZuAG transgenic plants. This supported that ZuAG is an AG homologue in Z. squash. Further functional analysis for ZuAG was also performed by cloning of full length and truncated cDNA for ZuAG into Zucchini yellow mosaic virus (ZYMV) infectious clone and introduced to zucchini plants. The female flowers were produced earlier in inoculated plants than in non inoculated plants. However, no obvious homeotic conversion for floral organs was observed in inoculated plants. |
| Original Identifier: | 089NCHU0408017 |
| نوع الوثيقة: | 學位論文 ; thesis |
| وصف الملف: | 112 |
| الإتاحة: | http://ndltd.ncl.edu.tw/handle/81243449555679816281Test |
| رقم الانضمام: | edsndl.TW.089NCHU0408017 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |