رسالة جامعية
Mesenchymal Analysis of Human Pluripotent Stem Cell-Derived Gastrointestinal Organoids
| العنوان: | Mesenchymal Analysis of Human Pluripotent Stem Cell-Derived Gastrointestinal Organoids |
|---|---|
| المؤلفون: | Haines, Lauren E. |
| بيانات النشر: | University of Cincinnati / OhioLINK, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | Ohiolink ETDs |
| Original Material: | http://rave.ohiolink.edu/etdc/view?acc_num=ucin1563873084551926Test |
| مصطلحات موضوعية: | Developmental Biology, mesenchyme, organoids, pluripotent stem cells, gut tube, endoderm |
| الوصف: | The gastrointestinal (GI) tract is an intricate and interconnected system vulnerable to dysfunction, with the National Institute of Health (NIH) estimating 60-70 million Americans across all demographics are affected by digestive disease each year (National Institutes of Health, 2009). This morbidity demands a biologically accurate model system to study the development and disease specific to the human GI tract with the ability of personalized medicine. We have used principles of GI organogenesis to direct the differentiation of human pluripotent stem cells into a variety of different organoids including foregut derived gastric organoids (HGOs) and midgut derived small intestinal organoids (HIOs). While all organoids contain epithelium, only HIOs contain a robust mesenchyme, which gives rise to essential cell types including smooth muscle and stroma. These mesenchymal derivates are critical for all organ function. Therefore, this research aims to gain insight into the mechanics of the in vitro mesenchyme contribution to GI tract organoids in order to improve the mesenchymal composition in foregut organoids. We have compared the gastric and intestinal organoid protocols and identified mesenchymal contribution is affected by BMP and Noggin/RA pathway manipulations during the formation of both definitive endoderm and gut tube stages. |
| Original Identifier: | oai:etd.ohiolink.edu:ucin1563873084551926 |
| نوع الوثيقة: | Text |
| اللغة: | English |
| الإتاحة: | http://rave.ohiolink.edu/etdc/view?acc_num=ucin1563873084551926Test |
| حقوق: | unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
| رقم الانضمام: | edsndl.OhioLink.oai.etd.ohiolink.edu.ucin1563873084551926 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |