دورية أكاديمية
Modeled microgravity unravels the roles of mechanical forces in renal progenitor cell physiology
| العنوان: | Modeled microgravity unravels the roles of mechanical forces in renal progenitor cell physiology |
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| المؤلفون: | Maria Elena Melica, Francesca Cialdai, Gilda La Regina, Chiara Risaliti, Tommaso Dafichi, Anna Julie Peired, Paola Romagnani, Monica Monici, Laura Lasagni |
| المصدر: | Stem Cell Research & Therapy, Vol 15, Iss 1, Pp 1-15 (2024) |
| بيانات النشر: | BMC, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Modeled microgravity, Renal progenitors, Podocyte, Cyclosporine A, Mechanobiology, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background The glomerulus is a highly complex system, composed of different interdependent cell types that are subjected to various mechanical stimuli. These stimuli regulate multiple cellular functions, and changes in these functions may contribute to tissue damage and disease progression. To date, our understanding of the mechanobiology of glomerular cells is limited, with most research focused on the adaptive response of podocytes. However, it is crucial to recognize the interdependence between podocytes and parietal epithelial cells, in particular with the progenitor subset, as it plays a critical role in various manifestations of glomerular diseases. This highlights the necessity to implement the analysis of the effects of mechanical stress on renal progenitor cells. Methods Microgravity, modeled by Rotary Cell Culture System, has been employed as a system to investigate how renal progenitor cells respond to alterations in the mechanical cues within their microenvironment. Changes in cell phenotype, cytoskeleton organization, cell proliferation, cell adhesion and cell capacity for differentiation into podocytes were analyzed. Results In modeled microgravity conditions, renal progenitor cells showed altered cytoskeleton and focal adhesion organization associated with a reduction in cell proliferation, cell adhesion and spreading capacity. Moreover, mechanical forces appeared to be essential for renal progenitor differentiation into podocytes. Indeed, when renal progenitors were exposed to a differentiative agent in modeled microgravity conditions, it impaired the acquisition of a complex podocyte-like F-actin cytoskeleton and the expression of specific podocyte markers, such as nephrin and nestin. Importantly, the stabilization of the cytoskeleton with a calcineurin inhibitor, cyclosporine A, rescued the differentiation of renal progenitor cells into podocytes in modeled microgravity conditions. Conclusions Alterations in the organization of the renal progenitor cytoskeleton due to unloading conditions negatively affect the regenerative capacity of these cells. These findings strengthen the concept that changes in mechanical cues can initiate a pathophysiological process in the glomerulus, not only altering podocyte actin cytoskeleton, but also extending the detrimental effect to the renal progenitor population. This underscores the significance of the cytoskeleton as a druggable target for kidney diseases. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| العلاقة: | https://doaj.org/toc/1757-6512Test |
| DOI: | 10.1186/s13287-024-03633-3 |
| الوصول الحر: | https://doaj.org/article/cfe994f7854b405abafed55e0a9103ccTest |
| رقم الانضمام: | edsdoj.fe994f7854b405abafed55e0a9103cc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-024-03633-3 |