دورية أكاديمية
Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells
العنوان: | Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells |
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المؤلفون: | Tomomi Mizokami, Hiroko Hisha, Satoshi Okazaki, Takashi Takaki, Xiao-Li Wang, Chang-Ye Song, Qing Li, Junko Kato, Naoki Hosaka, Muneo Inaba, Hideharu Kanzaki, Susumu Ikehara |
المصدر: | Haematologica, Vol 94, Iss 5 (2009) |
بيانات النشر: | Ferrata Storti Foundation, 2009. |
سنة النشر: | 2009 |
المجموعة: | LCC:Diseases of the blood and blood-forming organs |
مصطلحات موضوعية: | Diseases of the blood and blood-forming organs, RC633-647.5 |
الوصف: | Background We previously found in a murine hematopoietic system that hematopoietic stem cells show high differentiation and proliferation capacity on bone marrow-derived mesenchymal stem cells/stromal cells (microenvironment) with “self” major histocompatibility complex (MHC).Design and Methods We examined whether amnion-derived adherent cells have the characteristics of mesenchymal stem cells, and whether these adherent cells can support the proliferation of umbilical cord blood-derived lineage-negative and CD34-positive cells (Lin–CD34+ cells) obtained from the same fetus to a greater extent than those derived from other fetuses.Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could differentiate into osteoblasts, adipocytes and chondrocyte-like cells, indicating that the cells have the characteristics of mesenchymal stem cells. The Lin–CD34+ cells purified from the frozen umbilical cord blood were strongly positive for HLA-ABC, and contained a large number of hematopoietic stem cells. When the Lin–CD34+ cells were cultured on the autologous (MHC-matched) or MHC-mismatched amnion-derived adherent cells in short-term assays (hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin–CD34+ cells was observed in the MHC-matched combination than in MHC-mismatched combinations. The concentration of granulocyte-macrophage colony-stimulating factor in the culture supernatants of the long-term culture-initiating cell assays was significantly higher in the MHC-matched combination than in MHC-mismatched combinations.Conclusions It is likely that a MHC restriction exists between hematopoietic stem cells and mesenchymal stem cells/stromal cells in the human hematopoietic system and that granulocute-macropage colony-stimulating factor contributes to some extent to the preferential hematopoiesis-supporting ability of the MHC-matched amnion-derived adherent cells. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 0390-6078 1592-8721 |
العلاقة: | https://haematologica.org/article/view/5239Test; https://doaj.org/toc/0390-6078Test; https://doaj.org/toc/1592-8721Test |
DOI: | 10.3324/haematol.2008.004705 |
الوصول الحر: | https://doaj.org/article/ddfd22644d3c4ee485a490f642ebb3e7Test |
رقم الانضمام: | edsdoj.fd22644d3c4ee485a490f642ebb3e7 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 03906078 15928721 |
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DOI: | 10.3324/haematol.2008.004705 |