دورية أكاديمية
Regulation of gingival epithelial cytokine response by bacterial cyclic dinucleotides
| العنوان: | Regulation of gingival epithelial cytokine response by bacterial cyclic dinucleotides |
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| المؤلفون: | Samira Elmanfi, Jie Zhou, Herman O. Sintim, Eija Könönen, Mervi Gürsoy, Ulvi Kahraman Gürsoy |
| المصدر: | Journal of Oral Microbiology, Vol 11, Iss 1 (2019) |
| بيانات النشر: | Taylor & Francis Group, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | lipopolysaccharide, inflammation, periodontitis, interleukin, keratinocytes, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | Background: Cyclic dinucleotides (cyclic di-guanosine monophosphate (c-di-GMP) and cyclic di-adenosine monophosphate (c-di-AMP)) and lipopolysaccharides (LPS) are pathogen-associated molecular patterns (PAMPs). Individual impacts of PAMPs on immune system have been evaluated, but simultaneous actions of multiple PAMPs have not been studied. Objective: Examination the effects of cyclic dinucleotides and Porphyromonas gingivalis LPS on gingival epithelial cytokine response. Methods: Human gingival keratinocytes (HMK) were incubated with 1, 10, and 100 µM concentrations of c-di-GMP and c-di-AMP, either in the presence or absence of P. gingivalis LPS. Intra- and extracellular levels of interleukin (IL)-1β, IL-8, IL-1Ra, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF), were measured using the Luminex technique. Results: LPS decreased extracellular IL-8 levels, while the presence of c-di-AMP inhibited this effect. Incubating HMK cells with c-di-AMP (alone or with LPS) elevated the extracellular level of MCP-1. Extracellular VEGF level increased when cells were incubated with LPS and c-di-GMP together, or with c-di-AMP alone. LPS and c-di-AMP suppressed intracellular IL-1β levels. The c-di-AMP elevated intracellular levels of IL-1Ra. Conclusion: c-di-AMP and, to a lesser extent, c-di-GMP regulate keratinocyte cytokine response, either as an aggregator or as a suppressor of LPS, depending on the cytokine type. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2000-2297 20002297 |
| العلاقة: | https://doaj.org/toc/2000-2297Test |
| DOI: | 10.1080/20002297.2018.1538927 |
| الوصول الحر: | https://doaj.org/article/fcb302f2d0264251b1081c619765f99fTest |
| رقم الانضمام: | edsdoj.fcb302f2d0264251b1081c619765f99f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20002297 |
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| DOI: | 10.1080/20002297.2018.1538927 |