دورية أكاديمية
Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction
| العنوان: | Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction |
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| المؤلفون: | Jingbo Zhang, Junjun Li, Xiang Qu, Yuting Liu, Lifu Sun, Akima Harada, Ying Hua, Nagako Sougawa, Akiko Tabata, Li Liu, Shigeru Miyagawa |
| المصدر: | Bioactive Materials, Vol 37, Iss , Pp 533-548 (2024) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Biology (General) |
| مصطلحات موضوعية: | Human induced pluripotent stem cell-derived cardiomyocyte, Human adipose-derived mesenchymal stem cell, Tissue engineering, Myocardial infarction, Regenerative medicine, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5 |
| الوصف: | Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been widely used in therapy of ischemic heart disease. However, there are still remaining issues that limit the therapeutic efficacy, such as immune rejection and low retention of hiPSC-CMs. Human adipose mesenchymal stromal cells (hADSCs) have been reported to be able to regulate the immune response, promote angiogenesis and promote the maturation of hiPSC-CMs. In this study, we co-cultured these two types of cells on fiber scaffold made of biodegradable poly (D,L-lactic-co-glycolic acid) (PLGA) polymer for several days to develop a composited 3D cardiac tissue sheet. As expected, the cells formed 231.00 ± 15.14 μm thickness tissue, with improved organization, alignment, ECM condition, contractile ability, and paracrine function compared to culture hiPSC-CMs only on PLGA fiber. Furthermore, the composited 3D cardiac tissue sheet significantly promoted the engraftment and survival after transplantation. The composited 3D cardiac tissue sheet also increased cardiac function, attenuated ventricular remodeling, decreased fibrosis, and enhanced angiogenesis in rat myocardial infarction model, indicating that this strategy wound be a promising therapeutic option in the clinical scenario. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2452-199X |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2452199X24001099Test; https://doaj.org/toc/2452-199XTest |
| DOI: | 10.1016/j.bioactmat.2024.03.028 |
| الوصول الحر: | https://doaj.org/article/fb2d290dc6924a5588fb4b8e5ff48308Test |
| رقم الانضمام: | edsdoj.fb2d290dc6924a5588fb4b8e5ff48308 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2452199X |
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| DOI: | 10.1016/j.bioactmat.2024.03.028 |