دورية أكاديمية
Lost in transduction: Critical considerations when using viral vectors
العنوان: | Lost in transduction: Critical considerations when using viral vectors |
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المؤلفون: | William C. Hines |
المصدر: | Frontiers in Cell and Developmental Biology, Vol 10 (2023) |
بيانات النشر: | Frontiers Media S.A., 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Biology (General) |
مصطلحات موضوعية: | lentiviral vectors, viral transduction, multiplicity of infection (MOI), scientific reproducibility, cell barcoding, Biology (General), QH301-705.5 |
الوصف: | The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions—and describe how differential cell-type susceptibilities can confound results. We consider the math underlying this problem and review an alternative approach to the commonly used “multiplicity of infection” (MOI) method of titering and using viral vectors in the biomedical research laboratory. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2296-634X |
العلاقة: | https://www.frontiersin.org/articles/10.3389/fcell.2022.1080265/fullTest; https://doaj.org/toc/2296-634XTest |
DOI: | 10.3389/fcell.2022.1080265 |
الوصول الحر: | https://doaj.org/article/f9af0f463ae141c0900aa1c0e08fc4c7Test |
رقم الانضمام: | edsdoj.f9af0f463ae141c0900aa1c0e08fc4c7 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 2296634X |
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DOI: | 10.3389/fcell.2022.1080265 |