دورية أكاديمية
Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology
| العنوان: | Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology |
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| المؤلفون: | Aurelien Lathuiliere, Remi Vernet, Emily Charrier, Muriel Urwyler, Olivier Von Rohr, Marie-Claude Belkouch, Valentin Saingier, Thomas Bouvarel, Davy Guillarme, Adrien Engel, Patrick Salmon, Thomas Laumonier, Julien Grogg, Nicolas Mach |
| المصدر: | Molecular Therapy: Methods & Clinical Development, Vol 26, Iss , Pp 441-458 (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Genetics LCC:Cytology |
| مصطلحات موضوعية: | encapsulated cell technology, cell engineering, macroencapsulation, drug delivery, allogeneic cell transplant, myoblast, Genetics, QH426-470, Cytology, QH573-671 |
| الوصف: | Despite many promising results obtained in previous preclinical studies, the clinical development of encapsulated cell technology (ECT) for the delivery of therapeutic proteins from macrocapsules is still limited, mainly due to the lack of an allogeneic cell line compatible with therapeutic application in humans. In our work, we generated an immortalized human myoblast cell line specifically tailored for macroencapsulation. In the present report, we characterized the immortalized myoblasts and described the engineering process required for the delivery of functional therapeutic proteins including a cytokine, monoclonal antibodies and a viral antigen. We observed that, when encapsulated, the novel myoblast cell line can be efficiently frozen, stored, and thawed, which limits the challenge imposed by the manufacture and supply of encapsulated cell-based therapeutic products. Our results suggest that this versatile allogeneic cell line represents the next step toward a broader development and therapeutic use of ECT. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2329-0501 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2329050122001097Test; https://doaj.org/toc/2329-0501Test |
| DOI: | 10.1016/j.omtm.2022.07.017 |
| الوصول الحر: | https://doaj.org/article/adcf2f9abf0a4e4b9d902cb2e1d3ddbfTest |
| رقم الانضمام: | edsdoj.f2f9abf0a4e4b9d902cb2e1d3ddbf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23290501 |
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| DOI: | 10.1016/j.omtm.2022.07.017 |