دورية أكاديمية
Methods for multiplex template sampling in digital PCR assays.
| العنوان: | Methods for multiplex template sampling in digital PCR assays. |
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| المؤلفون: | Oleh I Petriv, Kevin A Heyries, Michael VanInsberghe, David Walker, Carl L Hansen |
| المصدر: | PLoS ONE, Vol 9, Iss 5, p e98341 (2014) |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing a 10-fold concentration enhancement while preserving assay precision. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | http://europepmc.org/articles/PMC4031183?pdf=renderTest; https://doaj.org/toc/1932-6203Test |
| DOI: | 10.1371/journal.pone.0098341 |
| الوصول الحر: | https://doaj.org/article/f017e679cf7a412685446e9e2a525aafTest |
| رقم الانضمام: | edsdoj.f017e679cf7a412685446e9e2a525aaf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0098341 |