دورية أكاديمية
Pygo-F773W Mutation Reveals Novel Functions beyond Wnt Signaling in Drosophila
| العنوان: | Pygo-F773W Mutation Reveals Novel Functions beyond Wnt Signaling in Drosophila |
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| المؤلفون: | Youfeng Li, Zhigang Jiang, Yue Xu, Jing Yan, Qiong Wu, Sirui Huang, Lingxiao Wang, Yulian Xie, Xiushan Wu, Yuequn Wang, Yongqing Li, Xiongwei Fan, Fang Li, Wuzhou Yuan |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 11, p 5998 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | Pygo, Wingless (Wg)/Wnt signaling pathway, CRISPR/Cas9, RNA-seq, point mutation, posttranslational modification, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Pygopus (Pygo) has been identified as a specific nuclear co-activator of the canonical Wingless (Wg)/Wnt signaling pathway in Drosophila melanogaster. Pygo proteins consist of two conserved domains: an N-terminal homologous domain (NHD) and a C-terminal plant homologous domain (PHD). The PHD’s ability to bind to di- and trimethylated lysine 4 of histone H3 (H3K4me2/3) appears to be independent of Wnt signaling. There is ongoing debate regarding the significance of Pygo’s histone-binding capacity. Drosophila Pygo orthologs have a tryptophan (W) > phenylalanine (F) substitution in their histone pocket-divider compared to vertebrates, leading to reduced histone affinity. In this research, we utilized CRISPR/Cas9 technology to introduce the Pygo-F773W point mutation in Drosophila, successfully establishing a viable homozygous Pygo mutant line for the first time. Adult mutant flies displayed noticeable abnormalities in reproduction, locomotion, heart function, and lifespan. RNA-seq and cluster analysis indicated that the mutation primarily affected pathways related to immunity, metabolism, and posttranslational modification in adult flies rather than the Wnt signaling pathway. Additionally, a reduction in H3K9 acetylation levels during the embryonic stage was observed in the mutant strains. These findings support the notion that Pygo plays a wider role in chromatin remodeling, with its involvement in Wnt signaling representing only a specific aspect of its chromatin-related functions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/25/11/5998Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms25115998 |
| الوصول الحر: | https://doaj.org/article/bed6bd7ca716426c8bfa14c75dc76363Test |
| رقم الانضمام: | edsdoj.bed6bd7ca716426c8bfa14c75dc76363 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25115998 |