دورية أكاديمية
Analysis of platelet eluate for the elucidation of sensitization to HLA in kidney transplant candidate
| العنوان: | Analysis of platelet eluate for the elucidation of sensitization to HLA in kidney transplant candidate |
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| المؤلفون: | Hugo Mendonça Mundim, Maria Aparecida Caixeta Lins, Eridane Botelho Guzmán, Heline Leal Titan, Patrícia Keiko Saito, Sueli Donizete Borelli |
| المصدر: | Acta Scientiarum. Health Sciences, Vol 37, Iss 2, Pp 175-179 (2015) |
| بيانات النشر: | Universidade Estadual de Maringá, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine (General) LCC:Pharmacy and materia medica |
| مصطلحات موضوعية: | antibodies, immunologic cytotoxicity tests, enzyme-linked immunosorbent assay, HLA Antigens, kidney transplantation, Medicine (General), R5-920, Pharmacy and materia medica, RS1-441 |
| الوصف: | While a 42-year-old male patient was being prepared for deceased-donor renal transplantation, anti-HLA-A2 antibodies were detected in the serum by enzyme-linked immunosorbent assay (ELISA) method. The patient denied any transfusion history and previous transplant. Crossmatch by complement dependent cytotoxicity (CDC) and CDC with anti-human globulin (CDC-AHG) proved negative with a four-cell panel with positive typing for HLA-A2. Adsorption of antibodies with platelets and analysis of eluate were suggested to elucidate discrepancies in results by ELISA and by CDC-AHG. ELISA showed that adsorbed serum with platelets did not reveal antibodies for HLA-A2 specificity and suggested that they were removed by their specific binding with HLA-A2 antigens on the platelet surface. Eluate analysis by ELISA showed antibodies for HLA-A2 specificity. No antibodies for HLA-A2 specificity in the non-adsorbed serum were detected by CDC-AHG method. Revision of patient’s data showed that a previous transfusion had occurred, which may have been the source of HLA sensitization. The suggested method may be a contribution towards the evaluation of sensitivity between CDC-AHG and ELISA methods for characterizing antibodies in the patient’s serum. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Portuguese |
| تدمد: | 1679-9291 1807-8648 |
| العلاقة: | http://186.233.154.254/ojs/index.php/ActaSciHealthSci/article/view/22127Test; https://doaj.org/toc/1679-9291Test; https://doaj.org/toc/1807-8648Test |
| DOI: | 10.4025/actascihealthsci.v37i2.22127 |
| الوصول الحر: | https://doaj.org/article/be1d98d94a0d46cea5403a11ed9a1ae2Test |
| رقم الانضمام: | edsdoj.be1d98d94a0d46cea5403a11ed9a1ae2 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16799291 18078648 |
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| DOI: | 10.4025/actascihealthsci.v37i2.22127 |