دورية أكاديمية
Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin
| العنوان: | Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin |
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| المؤلفون: | Renata Perotto de Souza, Vivian Favero, Vanessa Fey Pascoal, Catieli Lindholz, Hélio Radke Bittencourt, Carlos Graeff-Teixeira |
| المصدر: | Memorias do Instituto Oswaldo Cruz, Vol 114, Iss 0 |
| بيانات النشر: | Fundação Oswaldo Cruz (FIOCRUZ). |
| المجموعة: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | schistosomiasis, egg-detection, ninhydrin, coproparasitology, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eggs when applied in various combinations was also examined. Nine observers registered the presence or absence of 6 different criteria in 100 eggs using a microscope at 100x magnification. Ninhydrin purple, which was frequently observed, was the criterion associated with the lowest inter-observer variability. At least three criteria were associated with a significantly better performance in egg identification. In conclusion, ninhydrin staining and a combination of criteria are recommended for microscope examination of faecal sediments. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-8060 0074-0276 |
| العلاقة: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100409&lng=en&tlng=enTest; https://doaj.org/toc/1678-8060Test |
| DOI: | 10.1590/0074-02760180529 |
| الوصول الحر: | https://doaj.org/article/b760247709b942fab1f0b4b1879ee2c2Test |
| رقم الانضمام: | edsdoj.b760247709b942fab1f0b4b1879ee2c2 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16788060 00740276 |
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| DOI: | 10.1590/0074-02760180529 |