دورية أكاديمية
Unconventional function of an Achaete-Scute homolog as a terminal selector of nociceptive neuron identity.
العنوان: | Unconventional function of an Achaete-Scute homolog as a terminal selector of nociceptive neuron identity. |
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المؤلفون: | Neda Masoudi, Saeed Tavazoie, Lori Glenwinkel, Leesun Ryu, Kyuhyung Kim, Oliver Hobert |
المصدر: | PLoS Biology, Vol 16, Iss 4, p e2004979 (2018) |
بيانات النشر: | Public Library of Science (PLoS), 2018. |
سنة النشر: | 2018 |
المجموعة: | LCC:Biology (General) |
مصطلحات موضوعية: | Biology (General), QH301-705.5 |
الوصف: | Proneural genes are among the most early-acting genes in nervous system development, instructing blast cells to commit to a neuronal fate. Drosophila Atonal and Achaete-Scute complex (AS-C) genes, as well as their vertebrate orthologs, are basic helix-loop-helix (bHLH) transcription factors with such proneural activity. We show here that a C. elegans AS-C homolog, hlh-4, functions in a fundamentally different manner. In the embryonic, larval, and adult nervous systems, hlh-4 is expressed exclusively in a single nociceptive neuron class, ADL, and its expression in ADL is maintained via transcriptional autoregulation throughout the life of the animal. However, in hlh-4 null mutants, the ADL neuron is generated and still appears neuronal in overall morphology and expression of panneuronal and pansensory features. Rather than acting as a proneural gene, we find that hlh-4 is required for the ADL neuron to function properly, to adopt its correct morphology, to express its unusually large repertoire of olfactory receptor-encoding genes, and to express other known features of terminal ADL identity, including neurotransmitter phenotype, neuropeptides, ion channels, and electrical synapse proteins. hlh-4 is sufficient to induce ADL identity features upon ectopic expression in other neuron types. The expression of ADL terminal identity features is directly controlled by HLH-4 via a phylogenetically conserved E-box motif, which, through bioinformatic analysis, we find to constitute a predictive feature of ADL-expressed terminal identity markers. The lineage that produces the ADL neuron was previously shown to require the conventional, transient proneural activity of another AS-C homolog, hlh-14, demonstrating sequential activities of distinct AS-C-type bHLH genes in neuronal specification. Taken together, we have defined here an unconventional function of an AS-C-type bHLH gene as a terminal selector of neuronal identity and we speculate that such function could be reflective of an ancestral function of an "ur-" bHLH gene. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1544-9173 1545-7885 |
العلاقة: | http://europepmc.org/articles/PMC5908064?pdf=renderTest; https://doaj.org/toc/1544-9173Test; https://doaj.org/toc/1545-7885Test |
DOI: | 10.1371/journal.pbio.2004979 |
الوصول الحر: | https://doaj.org/article/b6e9e97fee494eaea422f48cd80b4a1bTest |
رقم الانضمام: | edsdoj.b6e9e97fee494eaea422f48cd80b4a1b |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 15449173 15457885 |
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DOI: | 10.1371/journal.pbio.2004979 |