دورية أكاديمية
Surface proteomics and label-free quantification of Leptospira interrogans serovar Pomona.
| العنوان: | Surface proteomics and label-free quantification of Leptospira interrogans serovar Pomona. |
|---|---|
| المؤلفون: | Teerasit Techawiwattanaboon, Praparat Thaibankluay, Chahya Kreangkaiwal, Suwitra Sathean-Anan-Kun, Prasong Khaenam, Jiradej Makjaroen, Trairak Pisitkun, Kanitha Patarakul |
| المصدر: | PLoS Neglected Tropical Diseases, Vol 15, Iss 11, p e0009983 (2021) |
| بيانات النشر: | Public Library of Science (PLoS), 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Public aspects of medicine |
| مصطلحات موضوعية: | Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270 |
| الوصف: | Leptospirosis is a re-emerging zoonosis with a global distribution. Surface-exposed outer membrane proteins (SE-OMPs) are crucial for bacterial-host interactions. SE-OMPs locate and expose their epitope on cell surface where is easily accessed by host molecules. This study aimed to screen for surface-exposed proteins and their abundance profile of pathogenic Leptospira interrogans serovar Pomona. Two complementary approaches, surface biotinylation and surface proteolytic shaving, followed by liquid chromatography tandem-mass spectrometry (LC-MS/MS) were employed to identify SE-OMPs of intact leptospires. For quantitative comparison, in-depth label-free analysis of SE-OMPs obtained from each method was performed using MaxQuant. The total number of proteins identified was 1,001 and 238 for surface biotinylation and proteinase K shaving, respectively. Among these, 39 were previously known SE-OMPs and 68 were predicted to be localized on the leptospiral surface. Based on MaxQuant analysis for relative quantification, six known SE-OMPs including EF- Tu, LipL21, LipL41, LipL46, Loa22, and OmpL36, and one predicted SE-OMPs, LipL71 were found in the 20 most abundant proteins, in which LipL41 was the highest abundant SE-OMP. Moreover, uncharacterized LIC14011 protein (LIP3228 ortholog in serovar Pomona) was identified as a novel predicted surface βb-OMP. High-abundance leptospiral SE-OMPs identified in this study may play roles in virulence and infection and are potential targets for development of vaccine or diagnostic tests for leptospirosis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1935-2727 1935-2735 |
| العلاقة: | https://doaj.org/toc/1935-2727Test; https://doaj.org/toc/1935-2735Test |
| DOI: | 10.1371/journal.pntd.0009983 |
| الوصول الحر: | https://doaj.org/article/b5210549fdcb484f81b5498b35650157Test |
| رقم الانضمام: | edsdoj.b5210549fdcb484f81b5498b35650157 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19352727 19352735 |
|---|---|
| DOI: | 10.1371/journal.pntd.0009983 |