دورية أكاديمية
Identification of novel cerebrospinal fluid biomarker candidates for dementia with Lewy bodies: a proteomic approach
| العنوان: | Identification of novel cerebrospinal fluid biomarker candidates for dementia with Lewy bodies: a proteomic approach |
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| المؤلفون: | Inger van Steenoven, Marleen J. A. Koel-Simmelink, Leonie J. M. Vergouw, Betty M. Tijms, Sander R. Piersma, Thang V. Pham, Claire Bridel, Gian-Luca Ferri, Cristina Cocco, Barbara Noli, Paul F. Worley, Mei-Fang Xiao, Desheng Xu, Patrick Oeckl, Markus Otto, Wiesje M. van der Flier, Frank Jan de Jong, Connie R. Jimenez, Afina W. Lemstra, Charlotte E. Teunissen |
| المصدر: | Molecular Neurodegeneration, Vol 15, Iss 1, Pp 1-15 (2020) |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Neurology. Diseases of the nervous system LCC:Geriatrics |
| مصطلحات موضوعية: | Biomarkers, Cerebrospinal fluid, Dementia with Lewy bodies, Lewy body dementia, Proteomics, Neurology. Diseases of the nervous system, RC346-429, Geriatrics, RC952-954.6 |
| الوصف: | Abstract Background Diagnosis of dementia with Lewy bodies (DLB) is challenging, largely due to a lack of diagnostic tools. Cerebrospinal fluid (CSF) biomarkers have been proven useful in Alzheimer’s disease (AD) diagnosis. Here, we aimed to identify novel CSF biomarkers for DLB using a high-throughput proteomic approach. Methods We applied liquid chromatography/tandem mass spectrometry with label-free quantification to identify biomarker candidates to individual CSF samples from a well-characterized cohort comprising patients with DLB (n = 20) and controls (n = 20). Validation was performed using (1) the identical proteomic workflow in an independent cohort (n = 30), (2) proteomic data from patients with related neurodegenerative diseases (n = 149) and (3) orthogonal techniques in an extended cohort consisting of DLB patients and controls (n = 76). Additionally, we utilized random forest analysis to identify the subset of candidate markers that best distinguished DLB from all other groups. Results In total, we identified 1995 proteins. In the discovery cohort, 69 proteins were differentially expressed in DLB compared to controls (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1750-1326 |
| العلاقة: | http://link.springer.com/article/10.1186/s13024-020-00388-2Test; https://doaj.org/toc/1750-1326Test |
| DOI: | 10.1186/s13024-020-00388-2 |
| الوصول الحر: | https://doaj.org/article/b358195c633d43b1b1052579bc71230bTest |
| رقم الانضمام: | edsdoj.b358195c633d43b1b1052579bc71230b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17501326 |
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| DOI: | 10.1186/s13024-020-00388-2 |