دورية أكاديمية
Dendritic cells mediated by small extracellular vesicles derived from MSCs attenuated the ILC2 activity via PGE2 in patients with allergic rhinitis
| العنوان: | Dendritic cells mediated by small extracellular vesicles derived from MSCs attenuated the ILC2 activity via PGE2 in patients with allergic rhinitis |
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| المؤلفون: | Xiao-Qing Liu, Ya-Qi Peng, Long-Xin Huang, Chan-Gu Li, Peng-Peng Kuang, De-Hua Chen, Zi-Cong Wu, Bi-Xin He, Zhi-Rou Zhou, Qing-Ling Fu |
| المصدر: | Stem Cell Research & Therapy, Vol 14, Iss 1, Pp 1-13 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Small extracellular vesicles, Mesenchymal stromal cells, Dendritic cells, Group 2 innate lymphoid cells, Prostaglandin E2, Allergic rhinitis, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Mesenchymal stromal cells-derived small extracellular vesicles (MSC-sEVs) have recently attracted considerable attention because of their therapeutic potential in various immune diseases. We previously reported that MSC-sEVs could exert immunomodulatory roles in allergic airway inflammation by regulating group 2 innate lymphoid cell (ILC2) and dendritic cell (DC) functions. Therefore, this study aimed to investigate the indirect effects of MSC-sEVs on ILC2s from patients with allergic rhinitis (AR) via DCs. Methods Here, we isolated sEVs from induced pluripotent stem cells-MSCs using anion-exchange chromatography and mature DCs (mDCs) were treated with MSC-sEVs. sEV-mDCs were co-cultured with peripheral blood mononuclear cells from patients with AR or purified ILC2s. The levels of IL-13 and GATA3 in ILC2s were examined by flow cytometry. Bulk RNA sequence for mDCs and sEV-mDCs was employed to further probe the potential mechanisms, which were then validated in the co-culture systems. Results sEV-mDCs showed impaired capacity in priming the levels of IL-13 and GATA3 in ILC2s when compared with mDCs. Furthermore, there was higher PGE2 and IL-10 production from sEV-mDCs, and the blockade of them especially the former one reversed the inhibitory effects of sEV-mDCs. Conclusions We demonstrated that MSC-sEVs were able to dampen the activating effects of mDCs on ILC2s in patients with AR. Mechanismly, the PGE2-EP2/4 axis played an essential role in the immunomodulatory effects of sEV-mDCs on ILC2s. Herein, we provided new insights into the mechanism underlying the therapeutic effects of MSC-sEVs in allergic airway inflammation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| العلاقة: | https://doaj.org/toc/1757-6512Test |
| DOI: | 10.1186/s13287-023-03408-2 |
| الوصول الحر: | https://doaj.org/article/c9f2ae35416b4a40815823b74aa66514Test |
| رقم الانضمام: | edsdoj.9f2ae35416b4a40815823b74aa66514 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-023-03408-2 |