دورية أكاديمية
α-Chitosan and β-Oligochitosan Mixtures-Based Formula for In Vitro Assessment of Melanocyte Cells Response
| العنوان: | α-Chitosan and β-Oligochitosan Mixtures-Based Formula for In Vitro Assessment of Melanocyte Cells Response |
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| المؤلفون: | Verginica Schröder, Daniela Gherghel, Manuela Rossemary Apetroaei, Cristiana Luminița Gîjiu, Raluca Isopescu, Daniel Dinculescu, Miruna-Maria Apetroaei, Laura Elena Enache, Cosmin-Teodor Mihai, Ileana Rău, Gabriela Vochița |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 12, p 6768 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | α-chitosan, β-oligochitosan, SK-MEL-28 melanocytes, YKL-40, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Chitosan is a natural polymer with numerous biomedical applications. The cellular activity of chitosan has been studied in various types of cancer, including melanoma, and indicates that these molecules can open new perspectives on antiproliferative action and anticancer therapy. This study analyzes how different chitosan conformations, such as α-chitosan (CH) or β-oligochitosan (CO), with various degrees of deacetylation (DDA) and molar mass (MM), both in different concentrations and in CH–CO mixtures, influence the cellular processes of SK-MEL-28 melanocytes, to estimate the reactivity of these cells to the applied treatments. The in vitro evaluation was carried out, aiming at the cellular metabolism (MTT assay), cellular morphology, and chitinase-like glycoprotein YKL-40 expression. The in vitro effect of the CH–CO mixture application on melanocytes is obvious at low concentrations of α-chitosan/β-oligochitosan (1:2 ratio), with the cell’s response supporting the hypothesis that β-oligo-chitosan amplifies the effect. This oligochitosan mixture, favored by the β conformation and its small size, penetrates faster into the cells, being more reactive when interacting with some cellular components. Morphological effects expressed by the loss of cell adhesion and the depletion of YKL-40 synthesis are significant responses of melanocytes. β-oligochitosan (1.5 kDa) induces an extension of cytophysiological effects and limits the cell viability compared to α-chitosan (400–900 kDa). Statistical analysis using multivariate techniques showed differences between the CH samples and CH–CO mixtures. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 25126768 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/25/12/6768Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms25126768 |
| الوصول الحر: | https://doaj.org/article/c9c6b87a479248e89459d9779cdb6de5Test |
| رقم الانضمام: | edsdoj.9c6b87a479248e89459d9779cdb6de5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25126768 14220067 16616596 |
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| DOI: | 10.3390/ijms25126768 |