دورية أكاديمية
Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats
| العنوان: | Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats |
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| المؤلفون: | Yuanyan LAI, Hongyun LI, Xiaowei MA, Zhihong JIANG, Jun WU |
| المصدر: | 环境与职业医学, Vol 40, Iss 1, Pp 89-94 (2023) |
| بيانات النشر: | Editorial Committee of Journal of Environmental and Occupational Medicine, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine (General) LCC:Toxicology. Poisons |
| مصطلحات موضوعية: | arsenic, hypothalamus, estrogen-like effect, thyrotropin-releasing hormone, thyrotropin-releasing hormone receptor, Medicine (General), R5-920, Toxicology. Poisons, RA1190-1270 |
| الوصف: | BackgroundArsenic can enter the hypothalamus to induce estrogen effect and interfere with the function of the neuroendocrine system. The thyroid endocrine system (hypothalamic-pituitary-thyroid axis) is one of the main endocrine systems, and the mechanism of arsenic-induced thyroid endocrine toxicity is still unclear. ObjectiveTo investigate the effects of different arsenic exposure levels on estradiol (E2), hypothalamic thyrotropin-releasing hormone (TRH), and their receptor (ERα, ERβ, and TRHR) mRNAs in rats and the possible hypothalamic toxic pathway and mechanism. MethodsSeventy Wister rats were randomly divided a control group (sterile water); low-, medium-, and high-dose arsenic exposure groups [0.8, 4.0, and 20.0 mg·kg−1 sodium arsenite (NaAsO2)]; estrogen receptor inhibitor (ICI182780) intervention + low-, medium-, and high-dose arsenic exposure groups; with 10 animals in each group, half male and half female. Rats in the arsenic exposure groups were exposed to NaAsO2 by drinking water for 19 weeks, and rats in the intervention groups were injected with 0.5 mg·kg−1 ICI182780 via tail vein at week 9, 3 times a week. The levels of E2 and TRH in serum of rats were detected by ELISA. The expression levels of estrogen receptor α (ERα), estrogen receptor β (ERβ), and TRH receptor (TRHR) mRNAs in hypothalamus of rats were detected by real-time PCR (RT-PCR). Results(1) E2 and its receptor mRNA: Compared with the control group, the serum E2 level of female rats was increased in the low-dose and the medium-dose arsenic exposure groups (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Chinese |
| تدمد: | 2095-9982 |
| العلاقة: | http://www.jeom.org/article/cn/10.11836/JEOM22274Test; https://doaj.org/toc/2095-9982Test |
| DOI: | 10.11836/JEOM22274 |
| الوصول الحر: | https://doaj.org/article/9ab8c7e2de894fd9806b3a6d69251a0fTest |
| رقم الانضمام: | edsdoj.9ab8c7e2de894fd9806b3a6d69251a0f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 20959982 |
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| DOI: | 10.11836/JEOM22274 |