دورية أكاديمية
أعرض في EDS

Golgi-targeting viscosity probe for the diagnosis of Alzheimer’s disease

التفاصيل البيبلوغرافية
العنوان: Golgi-targeting viscosity probe for the diagnosis of Alzheimer’s disease
المؤلفون: Wenjing Wu, Lingyu Zhao, Yuanyuan Zhang, Jinchao Wei, Juanjuan Han, Yangyang Zhang, Zhenwen Zhao
المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-11 (2024)
بيانات النشر: Nature Portfolio, 2024.
سنة النشر: 2024
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Abstract Early diagnosis and intervention of Alzheimer’s disease (AD) are particularly important to delay the pathological progression. Although fluorescent probes have been widely employed for investigating and diagnosing AD, their biological applications are significantly restricted due to the low penetration ability of the blood–brain barrier (BBB) in vivo. In this study, we reported the first Golgi-targeted two-photon (TP) fluorescent probe, DCM-DH, for detecting viscosity in the Golgi apparatus. The probe was rationally designed to exhibit superior analytical performance including high sensitivity, specific Golgi-targeting, efficient BBB penetration ability, and deep tissue penetration (247 μm) in the brains of AD model mice. Using the probe, we demonstrated that the fluorescence intensity in the human liver cancer cell (HepG2 cells) was higher than that of human normal liver cell (LO2 cells), and the brain viscosity of AD model mice increased significantly. We anticipate that this competent tool could be easily extended to other AD biomarkers for fundamental research on this detrimental disease.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
العلاقة: https://doaj.org/toc/2045-2322Test
DOI: 10.1038/s41598-023-50789-8
الوصول الحر: https://doaj.org/article/9aa3706b6a954551a01b796910248c0bTest
رقم الانضمام: edsdoj.9aa3706b6a954551a01b796910248c0b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-023-50789-8