دورية أكاديمية
Ubiquitous Luciferase Expression in 'Firefly Rats' Does Not Alter the Pancreatic Islet Morphology, Metabolism, and Function
| العنوان: | Ubiquitous Luciferase Expression in 'Firefly Rats' Does Not Alter the Pancreatic Islet Morphology, Metabolism, and Function |
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| المؤلفون: | Nelson Gonzalez, Hiroyuki Kato, Wilma Tixi, Jose Ortiz, Chris Orr, Hung-Ping Shih, Hsun Teresa Ku, Jiing-Kuan Yee, Fouad Kandeel, Yoko Mullen, Eiji Kobayashi, Hirotake Komatsu |
| المصدر: | Cell Transplantation, Vol 32 (2023) |
| بيانات النشر: | SAGE Publishing, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Medicine |
| الوصف: | “Firefly rats” ubiquitously express the luciferase reporter gene under the control of constitutively active ROSA26 promoter in inbred Lewis rats. Due to the minimal immunogenicity of luciferase, wide applications of Firefly rats have been reported in solid organ/cell transplantation studies for in vivo imaging, permitting quantitative and non-invasive tracking of the transplanted graft. ROSA26 is a non-coding gene and generally does not affect the expression of other endogenous genes. However, the effect of ubiquitous luciferase expression on islet morphology and function has not been thoroughly investigated, which is critical for the use of Firefly rats as islet donors in islet transplantation studies. Accordingly, in vivo glucose homeostasis (i.e., islet function in the native pancreas) was compared between age-matched luciferase-expressing Firefly rats and non-luciferase-expressing rats. In vivo assessments demonstrated no statistical difference between these rats in non-fasting blood glucose levels, intraperitoneal glucose tolerance tests, and glucose-stimulated serum C-peptide levels. Furthermore, islets were isolated from both rats to compare the morphology, function, and metabolism in vitro . Isolated islets from both rats exhibited similar in vitro characteristics in post-isolation islet yield, islet size, beta cell populations, insulin content per islet, oxygen consumption rate, and glucose-stimulated insulin secretion. In conclusion, ubiquitous luciferase expression in Firefly rats does not affect their islet morphology, metabolism, and function; this finding is critical and enables the use of isolated islets from Firefly rats for the dual assessment of islet graft function and bioluminescence imaging of islet grafts. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1555-3892 09636897 |
| العلاقة: | https://doaj.org/toc/1555-3892Test |
| DOI: | 10.1177/09636897231182497 |
| الوصول الحر: | https://doaj.org/article/d9939e4fbe0148df8740c480b1a26889Test |
| رقم الانضمام: | edsdoj.9939e4fbe0148df8740c480b1a26889 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15553892 09636897 |
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| DOI: | 10.1177/09636897231182497 |