دورية أكاديمية
Novel regulation of PKC-induced inflammation by Akt and protein phosphatase 2A in ovarian granulosa cells
| العنوان: | Novel regulation of PKC-induced inflammation by Akt and protein phosphatase 2A in ovarian granulosa cells |
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| المؤلفون: | Yen-Yu Lin, David Sun, Yuh-Lin Wu |
| المصدر: | Chinese Journal of Physiology, Vol 63, Iss 4, Pp 179-186 (2020) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Physiology |
| مصطلحات موضوعية: | akt, granulosa cell, phorbol 12-myristate 13-acetate, protein phosphatase 2a, Physiology, QP1-981 |
| الوصف: | PKC-mediated inflammation is important in ovarian physiology. The roles of Akt and protein phosphatase 2A (PP2A) in PKC-mediated inflammation in ovarian granulosa cells (GCs) remain mostly unclear. PKC activator phorbol 12-myristate 13-acetate induced the Akt phosphorylation in rat primary GCs but reduced the Akt phosphorylation in KGN human GCs. In rat GCs, an inhibitory effect of PI3K inhibitor wortmannin and a stimulatory effect of Akt activator SC79 on PKC-induced cyclooxygenase-2 (COX-2)/PGE2production were noted; wortmannin and SC79 acted oppositely in human GCs. In rat GCs, PP2A inhibitor okadaic acid further enhanced the PKC-mediated promoter activation and elevation of mRNA and protein levels of the COX-2 gene, whereas PP2A activator sodium selenate attenuated the PKC-mediated COX-2 expression and promoter activation. PKC activation did not affect PP2A phosphorylation, but okadaic acid indeed augmented the PKC-induced NF-κB nuclear translocation. Thus, PP2A appears to act as a negative modulator in PKC-mediated cellular inflammation in rat GCs, at least in part due to its attenuating effect on the PKC-induced NF-κB activation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0304-4920 2666-0059 |
| العلاقة: | http://www.cjphysiology.org/article.asp?issn=0304-4920;year=2020;volume=63;issue=4;spage=179;epage=186;aulast=LinTest; https://doaj.org/toc/0304-4920Test; https://doaj.org/toc/2666-0059Test |
| DOI: | 10.4103/CJP.CJP_44_20 |
| الوصول الحر: | https://doaj.org/article/936ee98216614ca7a3baa60e32f190aaTest |
| رقم الانضمام: | edsdoj.936ee98216614ca7a3baa60e32f190aa |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 03044920 26660059 |
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| DOI: | 10.4103/CJP.CJP_44_20 |