دورية أكاديمية
Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus
| العنوان: | Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus |
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| المؤلفون: | Shiori Sano, Shuetsu Fukushi, Souichi Yamada, Shizuko Harada, Hitomi Kinoshita, Satoko Sugimoto, Tomoki Yoshikawa, Takeshi Kurosu, Yuki Takamatsu, Masayuki Shimojima, Shoichi Toda, Yuka Hamada, Naoki Fujisawa, Takayuki Sugimoto, Masayuki Saijo |
| المصدر: | Viruses, Vol 13, Iss 4, p 693 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | SFTS virus, RT-LAMP, simplified method, Microbiology, QR1-502 |
| الوصف: | Detection of severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) during the early phase of the disease is important for appropriate treatment, infection control, and prevention of further transmission. The reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a nucleic acid amplification method that amplifies the target sequence under isothermal conditions. Here, we developed an RT-LAMP with a novel primer/probe set targeting a conserved region of the SFTSV L segment after extraction of viral RNA (standard RT-LAMP). Both the Chinese and Japanese SFTSV strains, including various genotypes, were detected by the standard RT-LAMP. We also performed RT-LAMP using the same primer/probe set but without the viral RNA extraction step (called simplified RT-LAMP) and evaluated the diagnostic efficacy. The sensitivity and specificity of the simplified RT-LAMP were 84.9% (45/53) and 89.5% (2/19), respectively. The simplified RT-LAMP can detect SFTSV in human sera containing >103.5 copies/mL viral RNA. The two RT-LAMP positive but quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) negative samples were positive in the conventional RT-PCR, suggesting that there was no false positive reaction in the RT-LAMP. Both the standard and simplified RT-LAMP are useful for detecting the SFTSV genome in patients during the early phase of the disease. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 13040693 1999-4915 |
| العلاقة: | https://www.mdpi.com/1999-4915/13/4/693Test; https://doaj.org/toc/1999-4915Test |
| DOI: | 10.3390/v13040693 |
| الوصول الحر: | https://doaj.org/article/d91a3cb6a59e4c7989ef6c0a17ca85e6Test |
| رقم الانضمام: | edsdoj.91a3cb6a59e4c7989ef6c0a17ca85e6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13040693 19994915 |
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| DOI: | 10.3390/v13040693 |