دورية أكاديمية
Effect of sciadopitysin on sodium arsenite-induced senescence of rat hippocampal neurons
| العنوان: | Effect of sciadopitysin on sodium arsenite-induced senescence of rat hippocampal neurons |
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| المؤلفون: | Cheng CHEN, Xiong CHEN, Aihua ZHANG |
| المصدر: | 环境与职业医学, Vol 39, Iss 5, Pp 550-555 (2022) |
| بيانات النشر: | Editorial Committee of Journal of Environmental and Occupational Medicine, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Toxicology. Poisons |
| مصطلحات موضوعية: | sodium arsenite, sciadopitysin, hippocampus, senescence of neurons, Medicine (General), R5-920, Toxicology. Poisons, RA1190-1270 |
| الوصف: | BackgroundIn addition to the typical signs of skin damage, long-term arsenic exposure is often accompanied by signs and symptoms of neurobehavioral abnormalities.ObjectiveTo investigate potential intervention effect of sciadopitysin on senescence of neurons induced by sodium arsenite in rats and possible underlying mediating effect of cell cycle-related transcription factor E2F1.MethodsSH-SY5Y cells were treated with 4 μmol·L−1 sodium arsenite for 24 h and intervened with 50 μg·mL−1 Ginkgo biloba extract (EGb761) or four major biflavonoids in Ginkgo biloba leaves (isoginkgetin, bilobetin, sciadopitysin, and ginkgetin) for 24 h respectively. Then, cell viability was measured by CCK-8 assay. Thirty-two 180-200 g SPF rats were randomly divided into a control group, an arsenic treatment group (10 mg·L−1), a Ginkgo biloba extract intervention group (10 mg·kg−1), and a sciadopitysin intervention group (10 mg·kg−1), 8 rats in each group, half male and half female. The rats were treated with sodium arsenite by free drinking water for 3 consecutive months, and the intervention treatment was conducted after 2 months of poisoning with drug intake by gavage for 1 month. HE staining was used to detect structural changes in the hippocampus, while Nissl's staining was used to detect changes in hippocampal morphology and neuron numbers. Moreover, senescence-associated β galactosidase (SA-β-gal) staining and Western blotting were used to detect senescence of hippocampal neurons and the expression level of E2F1, respectively.ResultsCompared to the arsenic treatment group, EGb761 and the four biflavonoids in Ginkgo biloba leaves effectively antagonized the inhibitory effect of sodium arsenite on cell viability (all Ps＜0.05), and sciadopitysin showed better restoration of cellular viability than Ginkgo biloba extract (P＜0.05). The results of HE staining and Nissl's staining showed that the hippocampal neurons in the arsenic treatment group were reduced in cell count and the synaptic structure was abnormal, with swelling, nuclear shrinkage, and vacuole, compared with the control group. The results of SA-β-gal staining showed that the number of senescent cells in the arsenic treatment group (15.75±3.01) was significantly increased compared with the control group (2.88±0.84) (P＜0.05); the numbers of senescent cells in the Ginkgo biloba extract group (9.38±1.92) and the sciadopitysin treatment group (7.75±2.38) were significantly decreased compared with the arsenic treatment group (all Ps＜0.05). The results of Western blotting showed that compared with the control group, the expression of E2F1 protein in hippocampus of the arsenic treatment group was significantly decreased (1.00±0.17 vs. 0.65±0.19, P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Chinese |
| تدمد: | 2095-9982 |
| العلاقة: | http://www.jeom.org/article/cn/10.11836/JEOM21587Test; https://doaj.org/toc/2095-9982Test |
| DOI: | 10.11836/JEOM21587 |
| الوصول الحر: | https://doaj.org/article/8888edf6726f4b4fb29d0f50710bb81cTest |
| رقم الانضمام: | edsdoj.8888edf6726f4b4fb29d0f50710bb81c |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 20959982 |
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| DOI: | 10.11836/JEOM21587 |