دورية أكاديمية
Study on proanthocyanidins crosslinked collagen membrane for guided bone tissue regeneration
| العنوان: | Study on proanthocyanidins crosslinked collagen membrane for guided bone tissue regeneration |
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| المؤلفون: | Hongfa Yang, Wai-Ching Liu, Xinrui Liu, Yunqian Li, Chingpo Lin, Yu-Min Lin, An-Ni Wang, Thu-Trang Nguyen, Yu-Chien Lin, Ren-Jei Chung |
| المصدر: | Journal of Applied Biomaterials & Functional Materials, Vol 19 (2021) |
| بيانات النشر: | SAGE Publishing, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | Biotechnology, TP248.13-248.65 |
| الوصف: | The goal of this study is to understand the ability of a newly developed barrier membrane to enhance bone tissue regeneration. Here in this study we present the in vitro characterization of the barrier membrane made from type I collagen and crosslinked by oligomeric proanthocyanidins (OPCs). The effects of the membrane (P-C film) on cell cycle, proliferation, alkaline phosphatase activity, and mineralization were evaluated using the human osteoblast cell line MG-63, while the barrier ability was examined using MG-63 cells, as well as the human skin fibroblast cell line WS-1. The pore size is one of the factors that plays a key role in tissue regeneration, therefore, we evaluated the pore size of the membrane using a capillary flow porometer. Our results showed that the mean pore size of the P-C film was approximately 7–9 µm, the size known to inhibit cell migration across the membrane. The P-C film also demonstrated excellent cell viability and good biocompatibility, since the cell number increased with time, with MG-63 cells proliferating faster on the P-C film than in the cell culture flask. Furthermore, the P-C film promoted osteoblast differentiation, resulting in higher alkaline phosphatase activity and mineralization. Therefore, our results suggest that this P-C film has a great potential to be used in guided bone regeneration during periodontal regeneration and bone tissue engineering. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2280-8000 22808000 |
| العلاقة: | https://doaj.org/toc/2280-8000Test |
| DOI: | 10.1177/22808000211005379 |
| الوصول الحر: | https://doaj.org/article/a87b85d125a14e89af1d367c179852b5Test |
| رقم الانضمام: | edsdoj.87b85d125a14e89af1d367c179852b5 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 22808000 |
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| DOI: | 10.1177/22808000211005379 |