دورية أكاديمية
أعرض في EDS

Linking single-cell measurements of mass, growth rate, and gene expression

التفاصيل البيبلوغرافية
العنوان: Linking single-cell measurements of mass, growth rate, and gene expression
المؤلفون: Robert J. Kimmerling, Sanjay M. Prakadan, Alejandro J. Gupta, Nicholas L. Calistri, Mark M. Stevens, Selim Olcum, Nathan Cermak, Riley S. Drake, Kristine Pelton, Frederik De Smet, Keith L. Ligon, Alex K. Shalek, Scott R. Manalis
المصدر: Genome Biology, Vol 19, Iss 1, Pp 1-13 (2018)
بيانات النشر: BMC, 2018.
سنة النشر: 2018
المجموعة: LCC:Biology (General)
LCC:Genetics
مصطلحات موضوعية: Single-cell RNA-Seq, Mass, Growth, Serial suspended microchannel resonator, Multi-omics, Single cell, Biology (General), QH301-705.5, Genetics, QH426-470
الوصف: Abstract Mass and growth rate are highly integrative measures of cell physiology not discernable via genomic measurements. Here, we introduce a microfluidic platform enabling direct measurement of single-cell mass and growth rate upstream of highly multiplexed single-cell profiling such as single-cell RNA sequencing. We resolve transcriptional signatures associated with single-cell mass and growth rate in L1210 and FL5.12 cell lines and activated CD8+ T cells. Further, we demonstrate a framework using these linked measurements to characterize biophysical heterogeneity in a patient-derived glioblastoma cell line with and without drug treatment. Our results highlight the value of coupled phenotypic metrics in guiding single-cell genomics.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1474-760X
العلاقة: http://link.springer.com/article/10.1186/s13059-018-1576-0Test; https://doaj.org/toc/1474-760XTest
DOI: 10.1186/s13059-018-1576-0
الوصول الحر: https://doaj.org/article/e86b5438fb3e4a98ad23b6bf3b2b5f02Test
رقم الانضمام: edsdoj.86b5438fb3e4a98ad23b6bf3b2b5f02
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1474760X
DOI:10.1186/s13059-018-1576-0