دورية أكاديمية
Factors affecting genotyping success in giant panda fecal samples
| العنوان: | Factors affecting genotyping success in giant panda fecal samples |
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| المؤلفون: | Ying Zhu, Hong-Yi Liu, Hai-Qiong Yang, Yu-Dong Li, He-Min Zhang |
| المصدر: | PeerJ, Vol 5, p e3358 (2017) |
| بيانات النشر: | PeerJ Inc., 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | Giant panda, Microsatellite fragment length, Long-term fecal DNA storage, Microsatellite base pair repeat unit, Storage time, Storage type, Medicine, Biology (General), QH301-705.5 |
| الوصف: | Fecal samples play an important role in giant panda conservation studies. Optimal preservation conditions and choice of microsatellites for giant panda fecal samples have not been established. In this study, we evaluated the effect of four factors (namely, storage type (ethanol (EtOH), EtOH −20 °C, 2-step storage medium, DMSO/EDTA/Tris/salt buffer (DETs) and frozen at −20 °C), storage time (one, three and six months), fragment length, and repeat motif of microsatellite loci) on the success rate of microsatellite amplification, allelic dropout (ADO) and false allele (FA) rates from giant panda fecal samples. Amplification success and ADO rates differed between the storage types. Freezing was inferior to the other four storage methods based on the lowest average amplification success and the highest ADO rates (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2167-8359 85746924 |
| العلاقة: | https://peerj.com/articles/3358.pdfTest; https://peerj.com/articles/3358Test/; https://doaj.org/toc/2167-8359Test |
| DOI: | 10.7717/peerj.3358 |
| الوصول الحر: | https://doaj.org/article/8574692410634047814b51c4e3e3e09aTest |
| رقم الانضمام: | edsdoj.8574692410634047814b51c4e3e3e09a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21678359 85746924 |
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| DOI: | 10.7717/peerj.3358 |