دورية أكاديمية
Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
| العنوان: | Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells |
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| المؤلفون: | Thatiana Corrêa de Melo, Dilza Trevisan-Silva, Miryam P. Alvarez-Flores, Renata Nascimento Gomes, Marcelo Medina de Souza, Hellen Paula Valerio, Douglas S. Oliveira, Carlos DeOcesano-Pereira, Viviane Fongaro Botosso, Soraia Attie Calil Jorge, Mirta Schattner, Ricardo M. Gomez, Ana Marisa Chudzinski-Tavassi |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 18, p 10452 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | SARS-CoV-2, endothelial cells, proteomics, mass spectrometry, HUVECs, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe respiratory failure. In severe cases, COVID-19 manifests as a thromboinflammatory disease. Damage to the vascular compartment caused by SARS-CoV-2 has been linked to thrombosis, triggered by an enhanced immune response. The molecular mechanisms underlying endothelial activation have not been fully elucidated. We aimed to identify the proteins correlated to the molecular response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2, which might help to unravel the molecular mechanisms of endothelium activation in COVID-19. In this direction, we exposed HUVECs to SARS-CoV-2 and analyzed the expression of specific cellular receptors, and changes in the proteome of HUVECs at different time points. We identified that HUVECs exhibit non-productive infection without cytopathic effects, in addition to the lack of expression of specific cell receptors known to be essential for SARS-CoV-2 entry into cells. We highlighted the enrichment of the protein SUMOylation pathway and the increase in SUMO2, which was confirmed by orthogonal assays. In conclusion, proteomic analysis revealed that the exposure to SARS-CoV-2 induced oxidative stress and changes in protein abundance and pathways enrichment that resembled endothelial dysfunction. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 85308536 |
| العلاقة: | https://www.mdpi.com/1422-0067/23/18/10452Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms231810452 |
| الوصول الحر: | https://doaj.org/article/85308536820c4380b3da93748640aac2Test |
| رقم الانضمام: | edsdoj.85308536820c4380b3da93748640aac2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 85308536 |
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| DOI: | 10.3390/ijms231810452 |