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Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway

التفاصيل البيبلوغرافية
العنوان: Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway
المؤلفون: Daisuke Miki, Peiying Zhu, Wencan Zhang, Yanfei Mao, Zhengyan Feng, Huan Huang, Hui Zhang, Yanqiang Li, Renyi Liu, Huiming Zhang, Yijun Qi, Jian-Kang Zhu
المصدر: Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
بيانات النشر: Nature Portfolio, 2017.
سنة النشر: 2017
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Abstract It has been reported that double-stranded break (DSB)-induced small RNAs (diRNAs) are generated via the RNA-directed DNA methylation pathway and function in DSB repair in Arabidposis. However, important questions remain regarding the biogenesis and function of diRNAs. Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to characterize diRNAs in Arabidopsis and rice. We found that 21-nt diRNAs were generated from a 35S promoter::GU-US reporter transgene targeted by CRISPR/Cas9. Unexpectedly, Pol II transcription of the transgene was required for efficient diRNA production and the level of diRNA accumulation correlated with the expression level of the transgene. diRNAs were not detected from CRISPR/Cas9- or TALEN-induced DSBs within the examined endogenous genes in Arabidopsis or rice. We also found that DCL4 and RDR6 that are known to be involved in posttranscriptional gene silencing were required to generate diRNAs. Our results suggest that DSBs are necessary but not sufficient for efficient diRNA generation and a high level of diRNAs is not necessary for DSB repair.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
العلاقة: https://doaj.org/toc/2045-2322Test
DOI: 10.1038/s41598-017-00374-7
الوصول الحر: https://doaj.org/article/832b4b6f88db40e49e7e863c3ee5b1acTest
رقم الانضمام: edsdoj.832b4b6f88db40e49e7e863c3ee5b1ac
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-017-00374-7