دورية أكاديمية
Non-genotoxic MDM2 inhibition selectively induces a pro-apoptotic p53 gene signature in chronic lymphocytic leukemia cells
العنوان: | Non-genotoxic MDM2 inhibition selectively induces a pro-apoptotic p53 gene signature in chronic lymphocytic leukemia cells |
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المؤلفون: | Carmela Ciardullo, Erhan Aptullahoglu, Laura Woodhouse, Wei-Yu Lin, Jonathan P Wallis, Helen Marr, Scott Marshall, Nick Bown, Elaine Willmore, John Lunec |
المصدر: | Haematologica, Vol 104, Iss 12 (2019) |
بيانات النشر: | Ferrata Storti Foundation, 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Diseases of the blood and blood-forming organs |
مصطلحات موضوعية: | Diseases of the blood and blood-forming organs, RC633-647.5 |
الوصف: | Chronic lymphocytic leukemia (CLL) is a clinically heterogeneous hematologic malignancy. In approximately 90% of cases the TP53 gene is in its wildtype state at diagnosis of this malignancy. As mouse double-minute-2 homolog (MDM2) is a primary repressor of p53, targeting this protein is an attractive therapeutic approach for non-genotoxic reactivation of p53. Since the discovery of the first MDM2 inhibitor, Nutlin-3a, newer potent and bioavailable compounds have been developed. In this study we tested the second-generation MDM2 inhibitor, RG7388, in patient-derived CLL cells and normal cells, examining its effect on the induction of p53-transcriptional targets. RG7388 potently decreased viability in p53-functional CLL cells, whereas p53-non-functional samples were more resistant to the drug. RG7388 induced a pro-apoptotic gene expression signature with upregulation of p53-target genes involved in the intrinsic (PUMA, BAX) and extrinsic (TNFRSF10B, FAS) pathways of apoptosis, as well as MDM2. Only a slight induction of CDKN1A was observed and upregulation of pro-apoptotic genes dominated, indicating that CLL cells are primed for p53-dependent apoptosis. Consequently, RG7388 led to a concentration-dependent increase in caspase-3/7 activity and cleaved poly (ADP-ribose) polymerase. Importantly, we observed a preferential pro-apoptotic signature in CLL cells but not in normal blood and bone marrow cells, including CD34+ hematopoietic cells. These data support the further evaluation of MDM2 inhibitors as a novel additional treatment option for patients with p53-functional CLL. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 0390-6078 1592-8721 |
العلاقة: | https://haematologica.org/article/view/9167Test; https://doaj.org/toc/0390-6078Test; https://doaj.org/toc/1592-8721Test |
DOI: | 10.3324/haematol.2018.206631 |
الوصول الحر: | https://doaj.org/article/7ecccf9c482b45a5821d7071778cdfacTest |
رقم الانضمام: | edsdoj.7ecccf9c482b45a5821d7071778cdfac |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 03906078 15928721 |
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DOI: | 10.3324/haematol.2018.206631 |