دورية أكاديمية
Identification of USP29 as a key regulator of nucleotide biosynthesis in neuroblastoma through integrative analysis of multi-omics data
| العنوان: | Identification of USP29 as a key regulator of nucleotide biosynthesis in neuroblastoma through integrative analysis of multi-omics data |
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| المؤلفون: | Ye Kang, Yahuan Yu, Yijia Liu, Yiwen Pan, Ru Zhang, Doudou Ren, Zeqiong Cai, Junpeng Ma, Xiaofan Xiong, Qi Zhang, Chengsheng Zhang, Rongfu Tu |
| المصدر: | Cancer Biology & Therapy, Vol 24, Iss 1 (2023) |
| بيانات النشر: | Taylor & Francis Group, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | usp29, cancer metabolism, nucleotide biosynthesis, neuroblastoma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Cancer cells show enhanced nucleotide biosynthesis, which is essential for their unlimited proliferation, but the underlying mechanisms are not entirely clear. Ubiquitin specific peptidase 29 (USP29) was reported to sustain neuroblastoma progression by promoting glycolysis and glutamine catabolism; however, its potential role in regulating nucleotide biosynthesis in tumor cells remains unknown. In this study, we depleted endogenous USP29 in MYCN-amplified neuroblastoma SK-N-BE2 cells by sgRNAs and conducted metabolomic analysis in cells with or without USP29 depletion, we found that USP29 deficiency caused a disorder of intermediates involved in glycolysis and nucleotide biosynthesis. De novo nucleotide biosynthesis was analyzed using 13C6 glucose as a tracer under normoxia and hypoxia. The results indicated that USP29-depleted cells showed inhibition of nucleotide anabolic intermediates derived from glucose, and this inhibition was more significant under hypoxic conditions. Analysis of RNA sequencing data in SK-N-BE2 cells demonstrated that USP29 promoted the gene expression of metabolic enzymes involved in nucleotide anabolism, probably by regulating MYC and E2F downstream pathways. These findings indicated that USP29 is a key regulator of nucleotide biosynthesis in tumor cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1538-4047 1555-8576 15384047 |
| العلاقة: | https://doaj.org/toc/1538-4047Test; https://doaj.org/toc/1555-8576Test |
| DOI: | 10.1080/15384047.2023.2237200 |
| الوصول الحر: | https://doaj.org/article/7d06242432c048e9b2de3e6d62442553Test |
| رقم الانضمام: | edsdoj.7d06242432c048e9b2de3e6d62442553 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15384047 15558576 |
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| DOI: | 10.1080/15384047.2023.2237200 |