دورية أكاديمية
LAP2α preserves genome integrity through assisting RPA deposition on damaged chromatin
| العنوان: | LAP2α preserves genome integrity through assisting RPA deposition on damaged chromatin |
|---|---|
| المؤلفون: | Kaiwen Bao, Qi Zhang, Shuai Liu, Nan Song, Qiushi Guo, Ling Liu, Shanshan Tian, Jihui Hao, Yi Zhu, Kai Zhang, Ding Ai, Jie Yang, Zhi Yao, Roland Foisner, Lei Shi |
| المصدر: | Genome Biology, Vol 23, Iss 1, Pp 1-22 (2022) |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | RPA loading, Genome stability, ATR activation, Homologous recombination, LAP2α, PARP1, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Abstract Background Single-stranded DNA (ssDNA) coated with replication protein A (RPA) acts as a key platform for the recruitment and exchange of genome maintenance factors in DNA damage response. Yet, how the formation of the ssDNA-RPA intermediate is regulated remains elusive. Results Here, we report that the lamin-associated protein LAP2α is physically associated with RPA, and LAP2α preferentially facilitates RPA deposition on damaged chromatin via physical contacts between LAP2α and RPA1. Importantly, LAP2α-promoted RPA binding to ssDNA plays a critical role in protection of replication forks, activation of ATR, and repair of damaged DNA. We further demonstrate that the preference of LAP2α-promoted RPA loading on damaged chromatin depends on poly ADP-ribose polymerase PARP1, but not poly(ADP-ribosyl)ation. Conclusions Our study provides mechanistic insight into RPA deposition in response to DNA damage and reveals a genome protection role of LAP2α. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1474-760X |
| العلاقة: | https://doaj.org/toc/1474-760XTest |
| DOI: | 10.1186/s13059-022-02638-6 |
| الوصول الحر: | https://doaj.org/article/78a997a021ef49149a9e7e12e686e10bTest |
| رقم الانضمام: | edsdoj.78a997a021ef49149a9e7e12e686e10b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1474760X |
|---|---|
| DOI: | 10.1186/s13059-022-02638-6 |