دورية أكاديمية
#264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development
| العنوان: | #264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development |
|---|---|
| المؤلفون: | Chungha Lee, Sangho Lee, Taeseop Shin, Geon Kim, Jieun Do, Jaephil Do, Ji Hyang Kim, Kyoung Hee Choi, Jae Young Kim, YongKeun Park |
| المصدر: | Fertility & Reproduction, Vol 05, Iss 04, Pp 430-431 (2023) |
| بيانات النشر: | World Scientific Publishing, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Reproduction |
| مصطلحات موضوعية: | Reproduction, QH471-489 |
| الوصف: | Background and Aims: Time-lapse technology is an emerging assisted reproductive technology (ART) that enables simultaneous culture and monitoring of embryos in vitro during their development. It provides extra information on key events during embryo development, which can assist embryologists in selecting embryos alongside traditional morphology evaluations. Various imaging technologies have been utilised to visualise and evaluate embryos such as brightfield microscopy, darkfield microscopy, and Hoffman modulation contrast microscopy. Although these imaging technologies allow for non-invasive imaging of human embryos in a clinical setting, 3D imaging of a whole embryo with subcellular resolution remains a challenge. Method: We demonstrate 3D time-lapse label-free imaging of live mouse embryos using the 3D refractive index (RI) imaging technique. The proposed method is a quantitative phase imaging technique that measures multiple-intensity images of transmitted light through the embryos via axial scanning to reconstruct the 3D RI map of the embryos. Results: Using the proposed method, live mouse embryos were monitored at different developmental stages, ranging from 2-cell embryos to expanded blastocysts. The measured 3D RI maps of the developing embryos reveal 3D landscape of subcellular features inside the embryos (Fig. 1). In the quantitative assessment of embryonic development, various morphokinematic parameters were extracted from the measured 3D RI maps of the embryos. Conclusion: Based on the results, we envision that our method has broad applications where non-invasive and quantitative assessment of embryonic development is required. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 26613182 2661-3174 2661-3182 |
| العلاقة: | https://doaj.org/toc/2661-3182Test; https://doaj.org/toc/2661-3174Test |
| DOI: | 10.1142/S2661318223742133 |
| الوصول الحر: | https://doaj.org/article/d781f939a05f4bf1945ec3c0d78ef240Test |
| رقم الانضمام: | edsdoj.781f939a05f4bf1945ec3c0d78ef240 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 26613182 26613174 |
|---|---|
| DOI: | 10.1142/S2661318223742133 |